Abstract
The paired helical filaments (PHFs) of Alzheimer's disease consist mainly of the microtubule-associated protein tau. PHF tau differs from normal human brain tau in that it has a higher Mr and a special state of phosphorylation. However, the protein kinase(s) involved, the phosphorylation sites on tau and the resulting conformational changes are only poorly understood. Here we show that a new monoclonal antibody, AT8, records the PHF-like state of tau in vitro, and we describe a kinase activity that turns normal tau into a PHF-like state. The epitope of AT8 is around residue 200, outside the region of internal repeats and requires the phosphorylation of serines 199 and/or 202. Both of these are followed by a proline, suggesting that the kinase activity belongs to the family of proline-directed kinases. The epitope of AT8 is nearly coincident with that of another phosphorylation-dependent antibody, TAU1 [Binder, L.I., Frankfurter, A. and Rebhun, L. (1985) J. Cell Biol., 101, 1371-1378], but the two are complementary since TAU1 requires a dephosphorylated epitope.
MeSH Terms
Alzheimer Disease/genetics,metabolism
Amino Acid Sequence
Animals
Binding Sites
Brain/metabolism
Cattle
Cloning, Molecular
Humans
Microtubules/metabolism
Molecular Sequence Data
Peptide Fragments/isolation & purification
Phosphopeptides/isolation & purification
Phosphorylation
Plasmids
Proline
Protein Kinases/metabolism
Serine
Swine
tau Proteins/genetics,metabolism
Chemicals
Peptide Fragments
Phosphopeptides
tau Proteins
Serine
Proline
Protein Kinases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Biernat J
Max-Planck-Unit for Structural Molecular Biology, Hamburg, FRG.
Mandelkow E M
Schröter C
Lichtenberg-Kraag B
Steiner B
Berling B
Meyer H
Mercken M
Vandermeeren A
Goedert M
Mandelkow E
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