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PMID: 15673716 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Noncoding DNA, isochores and gene expression: nucleosome formation potential.

Nucleic acids research ·Vol. 33 ·No. 2 ·2005-00-00 ·Pages 559-63

Vinogradov AE

Abstract

The nucleosome formation potential of introns, intergenic spacers and exons of human genes is shown here to negatively correlate with among-tissues breadth of gene expression. The nucleosome formation potential is also found to negatively correlate with the GC content of genomic sequences; the slope of regression line is steeper in exons compared with noncoding DNA (introns and intergenic spacers). The correlation with GC content is independent of sequence length; in turn, the nucleosome formation potential of introns and intergenic spacers positively (albeit weakly) correlates with sequence length independently of GC content. These findings help explain the functional significance of the isochores (regions differing in GC content) in the human genome as a result of optimization of genomic structure for epigenetic complexity and support the notion that noncoding DNA is important for orderly chromatin condensation and chromatin-mediated suppression of tissue-specific genes.

MeSH Terms
DNA, Intergenic/chemistry Exons GC Rich Sequence Gene Expression Genome, Human Humans Introns Isochores/chemistry Nucleosomes
Chemicals
DNA, Intergenic Isochores Nucleosomes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Vinogradov Alexander E
Institute of Cytology, Russian Academy of Sciences St Petersburg 194064, Russia. [email protected]
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki184
Email
[email protected]
Published
2005-00-00
Epub
2005-00-26
Pages
559-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC548339
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gki184 PMC Full Text View journal details
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