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PMID: 15750117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic relatedness of the new Matlab variants of Vibrio cholerae O1 to the classical and El Tor biotypes as determined by pulsed-field gel electrophoresis.

Journal of clinical microbiology ·Vol. 43 ·No. 3 ·2005-03-00 ·Pages 1401-4

Safa A, Bhuiyan NA, Alam M, Sack DA, Nair GB

Abstract

The genomes of the recently described Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes were compared with those of El Tor and classical biotypes by the use of pulsed-field gel electrophoresis. Dendrograms constructed using the unweighted-pair group method using average linkages generated from NotI restriction patterns of whole-chromosomal DNA grouped these strains into two major clusters that were found to be similar but not identical to those of either of the biotypes. Strains that clustered with the classical biotype appear to have been derived from the classical strains, which are thought to be extinct.

MeSH Terms
Bacterial Typing Techniques Electrophoresis, Gel, Pulsed-Field Genome, Bacterial Vibrio cholerae O1/classification,genetics
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Safa Ashrafus
Laboratory Sciences Division, ICDDR.B, GPO Box 128, Dhaka-1000, Bangladesh.
Bhuiyan Nurul Amin
Alam Munirul
Sack David A
Nair G Balakrish
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.43.3.1401-1404.2005
Published
2005-03-00
Pages
1401-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC1081274
Subset
IM
Grants
NIAID NIH HHS · R01 AI039129 · United States
External Links
PubMed source DOI: 10.1128/JCM.43.3.1401-1404.2005 PMC Full Text View journal details
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