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PMID: 7768818 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-O1 Vibrio cholerae.

Journal of bacteriology ·Vol. 177 ·No. 11 ·1995-06-00 ·Pages 3191-8

Karaolis DK, Lan R, Reeves PR

Abstract

The DNA sequences of the asd genes from 45 isolates of Vibrio cholerae (19 clinical O1 isolates, 2 environmental nontoxigenic O1 isolates, and 24 isolates with different non-O1 antigens) were determined. No differences were found within either sixth- or seventh-pandemic isolates; however, variation was found between the two forms and among the non-O1 isolates. O139 isolates had sequences identical to those of seventh-pandemic isolates. Phylogenetic trees with Vibrio mimicus as the outgroup suggest that the sixth-pandemic, seventh-pandemic, and U.S. Gulf isolates are three clones that have evolved independently from different lineages of environmental, nontoxigenic, non-O1 V. cholerae isolates. There is evidence for horizontal transfer of O antigen, since isolates with nearly identical asd sequences had different O antigens, and isolates with the O1 antigen did not cluster together but were found in different lineages. We also found evidence for recombination events within the asd gene of V. cholerae. V. cholerae may have a higher level of genetic exchange and a lower level of clonality than species such as Salmonella enterica and Escherichia coli.

Related Genes
asd
MeSH Terms
Bacterial Vaccines/immunology Base Sequence DNA Primers/chemistry Disease Outbreaks India Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Nucleic Acid United States Vibrio/classification,genetics,pathogenicity Vibrio cholerae/classification,genetics,pathogenicity
Chemicals
Bacterial Vaccines DNA Primers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Karaolis D K
Department of Microbiology (GO8), University of Sydney, New South Wales, Australia.
Lan R
Reeves P R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.11.3191-3198.1995
Published
1995-06-00
Pages
3191-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177010
Subset
IM
Databases
GENBANK
U25065, U25066, U25067, U25068, U25069, U25070, U25071, U25072, U25073, U25074, U25075, U25076, U25077, U25078, U25079, U25080, U25081, U25082, U25083, U25084, U25085, U25086, U25087
External Links
PubMed source DOI: 10.1128/jb.177.11.3191-3198.1995 PMC Full Text View journal details
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