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PMID: 15780142 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A gene expression fingerprint of C. elegans embryonic motor neurons.

BMC genomics ·Vol. 6 ·2005-03-21 ·Pages 42

Fox RM, Von Stetina SE, Barlow SJ, Shaffer C, Olszewski KL, Moore JH, Dupuy D, Vidal M, Miller DM

Abstract

Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo. Fluorescence Activated Cell Sorting (FACS) was used to isolate unc-4::GFP neurons from primary cultures of C. elegans embryonic cells. Microarray experiments detected 6,217 unique transcripts of which approximately 1,000 are enriched in unc-4::GFP neurons relative to the average nematode embryonic cell. The reliability of these data was validated by the detection of known cell-specific transcripts and by expression in UNC-4 motor neurons of GFP reporters derived from the enriched data set. In addition to genes involved in neurotransmitter packaging and release, the microarray data include transcripts for receptors to a remarkably wide variety of signaling molecules. The added presence of a robust array of G-protein pathway components is indicative of complex and highly integrated mechanisms for modulating motor neuron activity. Over half of the enriched genes (537) have human homologs, a finding that could reflect substantial overlap with the gene expression repertoire of mammalian motor neurons. We have described a microarray-based method, MAPCeL, for profiling gene expression in specific C. elegans motor neurons and provide evidence that this approach can reveal candidate genes for key roles in the differentiation and function of these cells. These methods can now be applied to generate a gene expression map of the C. elegans nervous system.

MeSH Terms
Animals Axons/metabolism Caenorhabditis elegans Cell Differentiation Cell Movement Cell Separation Databases, Genetic Flow Cytometry Gene Expression Profiling/methods Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins/metabolism Microscopy, Fluorescence Models, Biological Motor Neurons/metabolism Neurons/metabolism Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic RNA/metabolism Receptors, Nicotinic/metabolism Signal Transduction Transgenes
Chemicals
Receptors, Nicotinic Green Fluorescent Proteins RNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Fox Rebecca M
Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232-8240, USA. [email protected]
Von Stetina Stephen E
Barlow Susan J
Shaffer Christian
Olszewski Kellen L
Moore Jason H
Dupuy Denis
Vidal Marc
Miller David M
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Article Info
Journal
BMC genomics
Abbr.
BMC Genomics
ISSN
1471-2164
DOI
10.1186/1471-2164-6-42
Email
[email protected]
Published
2005-03-21
Epub
2005-00-21
Pages
42
Language
English
Region
England
NLM ID
100965258
PMCID
PMC1079822
Subset
IM
Grants
NIDDK NIH HHS · DK58749 · United States
NIDDK NIH HHS · P01 DK58212 · United States
NICHD NIH HHS · T32 HD007502 · United States
NIDDK NIH HHS · P60 DK020593 · United States
NINDS NIH HHS · F31 NS043068 · United States
NEI NIH HHS · P30 EY08126 · United States
NIDDK NIH HHS · DK58404 · United States
NINDS NIH HHS · R01 NS026115 · United States
NCI NIH HHS · P30 CA68485 · United States
NIDDK NIH HHS · P30 DK058404 · United States
NICHD NIH HHS · P30 HD015052 · United States
NHLBI NIH HHS · 1 P01 HL6744-01 · United States
NEI NIH HHS · EY08126 · United States
NEI NIH HHS · P30 EY008126 · United States
NINDS NIH HHS · F31 NS046923 · United States
NIDDK NIH HHS · P30 DK020593 · United States
NCI NIH HHS · P30 CA068485 · United States
NCI NIH HHS · CA68485 · United States
NIDDK NIH HHS · P60 DK20593 · United States
NICHD NIH HHS · HD15052 · United States
NIDDK NIH HHS · DK20593 · United States
NIDDK NIH HHS · P30 DK58404 · United States
NCI NIH HHS · R33 CA097516 · United States
NINDS NIH HHS · R01 NS26115 · United States
NIDDK NIH HHS · P01 DK058212 · United States
External Links
PubMed source DOI: 10.1186/1471-2164-6-42 PMC Full Text View journal details
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