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PMID: 15824315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 16 ·2005-04-19 ·Pages 5733-8

Li J, Krichevsky A, Vaidya M, Tzfira T, Citovsky V

Abstract

Agrobacterium-mediated genetic transformation of plants, a unique example of transkingdom DNA transfer, requires the presence of several proteins encoded by the host cell. One such cellular factor is VIP1, an Arabidopsis protein proposed to interact with and facilitate import of the bacterial DNA-protein transport (T) complexes into the plant cell nucleus. Thus, VIP1 is required for transient expression of the bacterial DNA, an early step in the transformation process. However, the role of VIP1 in subsequent transformation events leading to the stable expression of bacterial DNA was unexplored. Here, we used reverse genetics to dissect VIP1 functionally and demonstrate its involvement in the stable genetic transformation of Arabidopsis plants by Agrobacterium. Our data indicate that the ability of VIP1 to interact with the VirE2 protein component of the T-complex and localize to the cell nucleus is sufficient for transient genetic transformation, whereas its ability to form homomultimers and interact with the host cell H2A histone in planta is required for tumorigenesis and, by implication, stable genetic transformation.

MeSH Terms
Active Transport, Cell Nucleus Amino Acid Sequence Animals Arabidopsis/genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence Cell Nucleus/metabolism DNA-Binding Proteins/genetics,metabolism Genetic Complementation Test Ion Channels/genetics,metabolism Luminescent Proteins/genetics,metabolism Molecular Sequence Data Multiprotein Complexes Phenotype Protein Structure, Quaternary Rhizobium/genetics,metabolism Transformation, Genetic
Chemicals
Arabidopsis Proteins Bacterial Proteins DNA-Binding Proteins Ion Channels Luminescent Proteins Multiprotein Complexes VIP1 protein, Arabidopsis virE2 protein, Agrobacterium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li Jianxiong
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA.
Krichevsky Alexander
Vaidya Manjusha
Tzfira Tzvi
Citovsky Vitaly
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0404118102
Published
2005-04-19
Epub
2005-00-11
Pages
5733-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC556277
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0404118102 PMC Full Text View journal details
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