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PMID: 15843961 Published · ppublish English Journal Article

Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants.

Planta ·Vol. 222 ·No. 1 ·2005-09-00 ·Pages 192-200

Kuzniak E, Skłodowska M

Abstract

Peroxisomes, being one of the main organelles where reactive oxygen species (ROS) are both generated and detoxified, have been suggested to be instrumental in redox-mediated plant cell defence against oxidative stress. We studied the involvement of tomato (Lycopersicon esculentum Mill.) leaf peroxisomes in defence response to oxidative stress generated upon Botrytis cinerea Pers. infection. The peroxisomal antioxidant potential expressed as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) and glutathione peroxidase (GSH-Px, EC 1.11.1.19) as well as the ascorbate-glutathione (AA-GSH) cycle activities was monitored. The initial infection-induced increase in SOD, CAT and GSH-Px indicating antioxidant defence activation was followed by a progressive inhibition concomitant with disease symptom development. Likewise, the activities of AA-GSH cycle enzymes: ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1) and glutathione reductase (GR, EC 1.6.4.2) as well as ascorbate and glutathione concentrations and redox ratios were significantly decreased. However, the rate and timing of these events differed. Our results indicate that B. cinerea triggers significant changes in the peroxisomal antioxidant system leading to a collapse of the protective mechanism at advanced stage of infection. These changes appear to be partly the effect of pathogen-promoted leaf senescence.

MeSH Terms
Antioxidants/metabolism Ascorbate Peroxidases Ascorbic Acid/analogs & derivatives,metabolism Botrytis/physiology Catalase/metabolism Chlorophyll/analysis Glutathione Peroxidase/metabolism Lycopersicon esculentum/cytology,metabolism,microbiology NADH, NADPH Oxidoreductases/metabolism Oxidative Stress Peroxidases/metabolism Peroxisomes/enzymology,metabolism,microbiology Plant Diseases/microbiology Plant Leaves/cytology,metabolism,microbiology Reactive Oxygen Species/metabolism Superoxide Dismutase/metabolism Time Factors
Chemicals
Antioxidants Reactive Oxygen Species Chlorophyll Peroxidases Ascorbate Peroxidases Catalase Glutathione Peroxidase Superoxide Dismutase NADH, NADPH Oxidoreductases monodehydroascorbate reductase (NADH) Ascorbic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuzniak Elźbieta
Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland. [email protected]
Skłodowska Maria
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-005-1514-8
Email
[email protected]
Published
2005-09-00
Epub
2005-00-21
Pages
192-200
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-005-1514-8 View journal details
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