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PMID: 15886323 Published · ppublish English Journal Article

Wnts induce migration and invasion of myeloma plasma cells.

Blood ·Vol. 106 ·No. 5 ·2005-09-01 ·Pages 1786-93

Qiang YW, Walsh K, Yao L, Kedei N, Blumberg PM, Rubin JS, Shaughnessy J, Rudikoff S

Abstract

Multiple myeloma is an incurable form of lymphoid cancer characterized by accumulation of neoplastic plasma cells in the bone marrow cavity. Little is known about the mechanisms regulating myeloma cell movement within the bone marrow and metastasis to secondary sites. Herein, we identify multiple members of the wingless/int (Wnt) family as promoters of myeloma cell migration/invasion. Wnt-mediated migration was associated with the Wnt/RhoA pathway and did not necessitate signaling through beta-catenin. Activation of both RhoA and members of the protein kinase C (PKC) family, including PKCalpha, PKCbeta, and PKCmu, were required for induction of migration. Activated RhoA and PKCalpha, PKCbeta, and PKCmu appear to assemble in macromolecular signaling complexes that are associated with the cell membrane. These results suggest that Wnt responsiveness of myeloma plasma cells may be a significant factor in disease progression.

MeSH Terms
Cell Line, Tumor Cell Movement/drug effects,physiology Humans Intercellular Signaling Peptides and Proteins/pharmacology,physiology Macromolecular Substances/metabolism Multiple Myeloma/metabolism,pathology Neoplasm Invasiveness/physiopathology Protein Kinase C/physiology Signal Transduction/drug effects,physiology Wnt Proteins rhoA GTP-Binding Protein/metabolism
Chemicals
Intercellular Signaling Peptides and Proteins Macromolecular Substances Wnt Proteins Protein Kinase C rhoA GTP-Binding Protein
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Qiang Ya-Wei
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Walsh Katie
Yao Lei
Kedei Noemi
Blumberg Peter M
Rubin Jeffrey S
Shaughnessy John
Rudikoff Stuart
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2005-01-0049
Published
2005-09-01
Epub
2005-00-10
Pages
1786-93
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1895227
Subset
IM
External Links
PubMed source DOI: 10.1182/blood-2005-01-0049 PMC Full Text View journal details
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