Home LiteratureArticle Details
PMID: 15902490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the melon genome in regions encompassing TIR-NBS-LRR resistance genes.

Molecular genetics and genomics : MGG ·Vol. 273 ·No. 3 ·2005-05-00 ·Pages 240-51

van Leeuwen H, Garcia-Mas J, Coca M, Puigdoménech P, Monfort A

Abstract

Plant genomes contain numerous genes (R-genes) that play a role in initiating defence measures against their particular pathogens. Defence mechanisms controlled by R-genes have been the focus of extensive research over the past several years. The majority of the R-genes described so far belong to a super-family of genes (150-600 members) that encode proteins with a nucleotide binding site (NBS), some leucine-rich repeats (LRR) and an N-terminal domain that shows similarity to the Toll and Interleukin-1 receptors (TIR) or a N-terminal coiled-coil (CC) domain. Analysis of four regions of the melon (Cucumis melo) genome, including two sequenced BACs, identified 14 TIR-NBS-LRR genes. Known disease resistance genes have been mapped in three of these regions. Transcriptional expression was detected for predicted genes that are possibly involved in defence responses to pathogen attack. TIR-NBS-LRR genes appear to be clustered in the melon genome. They contain all the conserved motifs that have previously been described for their counterparts in other species, although differences were also detected. The results presented here may contribute to a better understanding of the genomic distribution and evolution of this group of resistance gene homologues and their variability.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Artificial, Bacterial Cloning, Molecular Cucurbitaceae/genetics,microbiology DNA Primers Genome, Plant Molecular Sequence Data Phylogeny Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA Primers
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Leeuwen Hans
Laboratori de Genética Molecular Vegetal, Consorci CSIC-IRTA, Barcelona, Spain.
Garcia-Mas Jordi
Coca María
Puigdoménech Pere
Monfort Amparo
References (39)
39 references, click to expand
  1. The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats.
    Cell. 1994 Sep 23;78(6):1089-99 PMID: 7923358
  2. JDotter: a Java interface to multiple dotplots generated by dotter.
    Bioinformatics. 2004 Jan 22;20(2):279-81 PMID: 14734323
  3. Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily.
    Plant J. 1999 Nov;20(3):317-32 PMID: 10571892
  4. Consed: a graphical tool for sequence finishing.
    Genome Res. 1998 Mar;8(3):195-202 PMID: 9521923
  5. Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process.
    Genome Res. 1998 Nov;8(11):1113-30 PMID: 9847076
  6. Improved method for the isolation of RNA from plant tissues.
    Anal Biochem. 1987 May 15;163(1):16-20 PMID: 2441623
  7. Phylogeny and genomic organization of the TIR and non-tIR NBS-LRR resistance gene family in Medicago truncatula.
    Mol Plant Microbe Interact. 2002 Jun;15(6):529-39 PMID: 12059101
  8. Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana.
    Plant Mol Biol. 2003 Mar;51(5):703-18 PMID: 12678558
  9. Construction of a reference linkage map for melon.
    Genome. 2001 Oct;44(5):836-45 PMID: 11681608
  10. Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding.
    Curr Opin Biotechnol. 2003 Apr;14(2):177-93 PMID: 12732319
  11. Transposon diversity in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7376-81 PMID: 10861007
  12. Genetic and physical localization of the soybean Rpg1-b disease resistance gene reveals a complex locus containing several tightly linked families of NBS-LRR genes.
    Mol Plant Microbe Interact. 2003 Sep;16(9):817-26 PMID: 12971605
  13. Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes.
    Mol Genet Genomics. 2004 May;271(4):402-15 PMID: 15014983
  14. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential.
    Genome Res. 2001 Aug;11(8):1441-52 PMID: 11483586
  15. Intragenic recombination generated two distinct Cf genes that mediate AVR9 recognition in the natural population of Lycopersicon pimpinellifolium.
    Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10493-8 PMID: 11517316
  16. Human BAC library: construction and rapid screening.
    Gene. 1997 May 20;191(1):69-79 PMID: 9210591
  17. BCM Search Launcher--an integrated interface to molecular biology data base search and analysis services available on the World Wide Web.
    Genome Res. 1996 May;6(5):454-62 PMID: 8743995
  18. Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance gene.
    Trends Genet. 2004 Mar;20(3):116-22 PMID: 15049302
  19. Regions outside of the leucine-rich repeats of flax rust resistance proteins play a role in specificity determination.
    Plant Cell. 2000 Aug;12(8):1367-77 PMID: 10948256
  20. Mode of amplification and reorganization of resistance genes during recent Arabidopsis thaliana evolution.
    Mol Biol Evol. 2002 Jan;19(1):76-84 PMID: 11752192
  21. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato.
    Cell. 1997 Dec 12;91(6):821-32 PMID: 9413991
  22. Identification of quantitative trait loci involved in fruit quality traits in melon (Cucumis melo L.).
    Theor Appl Genet. 2004 Feb;108(4):750-8 PMID: 14576985
  23. Isolation and characterization of a full-length resistance gene homolog from soybean.
    Theor Appl Genet. 2003 Mar;106(5):786-93 PMID: 12647051
  24. Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity.
    Plant Cell. 1999 Mar;11(3):495-506 PMID: 10072407
  25. The genetic architecture of resistance.
    Curr Opin Plant Biol. 2000 Aug;3(4):285-90 PMID: 10873848
  26. Angiosperm phylogeny inferred from multiple genes as a tool for comparative biology.
    Nature. 1999 Nov 25;402(6760):402-4 PMID: 10586878
  27. Rapid induction of a protein disulfide isomerase and defense-related genes in wheat in response to the hemibiotrophic fungal pathogen Mycosphaerella graminicola.
    Plant Mol Biol. 2003 Nov;53(5):701-14 PMID: 15010608
  28. Sequence architecture downstream of the initiator codon enhances gene expression and protein stability in plants.
    Plant Physiol. 2001 Aug;126(4):1630-6 PMID: 11500561
  29. TreeView: an application to display phylogenetic trees on personal computers.
    Comput Appl Biosci. 1996 Aug;12(4):357-8 PMID: 8902363
  30. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  31. RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes.
    Science. 1994 Sep 23;265(5180):1856-60 PMID: 8091210
  32. Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis.
    Plant Cell. 2003 Apr;15(4):809-34 PMID: 12671079
  33. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  34. Distribution of microsatellites in relation to coding sequences within the Arabidopsis thaliana genome.
    Plant Sci. 2000 Aug 8;157(1):97-104 PMID: 10940473
  35. Speculations on RNA splicing.
    Cell. 1981 Mar;23(3):643-6 PMID: 7226224
  36. The Staden sequence analysis package.
    Mol Biotechnol. 1996 Jun;5(3):233-41 PMID: 8837029
  37. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  38. The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response.
    Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15849-54 PMID: 9861059
  39. The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor.
    Cell. 1994 Sep 23;78(6):1101-15 PMID: 7923359
Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-004-1104-7
Published
2005-05-00
Epub
2005-00-12
Pages
240-51
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-004-1104-7 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2