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PMID: 15923605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential requirement of SAGA subunits for Mot1p and Taf1p recruitment in gene activation.

Molecular and cellular biology ·Vol. 25 ·No. 12 ·2005-06-00 ·Pages 4863-72

van Oevelen CJ, van Teeffelen HA, Timmers HT

Abstract

Transcription activation in yeast (Saccharomyces cerevisiae) involves ordered recruitment of transcription factor complexes, such as TFIID, SAGA, and Mot1p. Previously, we showed that both Mot1p and Taf1p are recruited to the HXT2 and HXT4 genes, which encode hexose transporter proteins. Here, we show that SAGA also binds to the HXT2 and HXT4 promoters and plays a pivotal role in the recruitment of Mot1p and Taf1p. The deletion of either SPT3 or SPT8 reduces Mot1p binding to HXT2 and HXT4. Surprisingly, the deletion of GCN5 reduces Taf1p binding to both promoters. When GCN5 is deleted in spt3Delta or spt8Delta strains, neither Mot1p nor Taf1p binds, and this results in a diminished recruitment of TATA binding protein and polymerase II to the HXT4 but not the HXT2 promoter. This is reflected by the SAGA-dependent expression of HXT4. In contrast, SAGA-independent induction of HXT2 suggests a functional redundancy with other factors. A functional interplay of different SAGA subunits with Mot1p and Taf1p was supported by phenotypic analysis of MOT1 SAGA or TAF1/SAGA double mutant strains, which revealed novel genetic interactions between MOT1 and SPT8 and between TAF1 and GCN5. In conclusion, our data demonstrate functional links between SAGA, Mot1p, and TFIID in HXT gene regulation.

MeSH Terms
Adenosine Triphosphatases DNA Helicases/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Fungal Glucose/metabolism Glucose Transport Proteins, Facilitative Histone Acetyltransferases Membrane Proteins/genetics,metabolism Monosaccharide Transport Proteins/genetics,metabolism Promoter Regions, Genetic Protein Binding Protein Kinases/genetics,metabolism Protein Subunits/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism TATA-Binding Protein Associated Factors/genetics,metabolism Transcription Factor TFIID/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic Transcriptional Activation
Chemicals
DNA-Binding Proteins Glucose Transport Proteins, Facilitative HXT2 protein, S cerevisiae HXT4 protein, S cerevisiae Membrane Proteins Monosaccharide Transport Proteins Protein Subunits SPT3 protein, S cerevisiae SPT8 protein, S cerevisiae Saccharomyces cerevisiae Proteins TAF1 protein, S cerevisiae TATA-Binding Protein Associated Factors Transcription Factor TFIID Transcription Factors GCN5 protein, S cerevisiae Histone Acetyltransferases Protein Kinases Adenosine Triphosphatases MOT1 protein, S cerevisiae DNA Helicases Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Oevelen Chris J C
Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
van Teeffelen Hetty A A M
Timmers H T Marc
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.12.4863-4872.2005
Published
2005-06-00
Pages
4863-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1140607
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.25.12.4863-4872.2005 PMC Full Text View journal details
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