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PMID: 1598203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The C terminus of the NF-kappa B p50 precursor and an I kappa B isoform contain transcription activation domains.

Nucleic acids research ·Vol. 20 ·No. 10 ·1992-05-25 ·Pages 2453-8

Morin PJ, Gilmore TD

Abstract

The p50 subunit of the NF-kappa B transcription complex is derived from the N-terminal half of a larger precursor protein, p105. Although a fair amount is known about functions located within the p50 sequences, less is known about the C-terminal half of p105. In this report, we have identified a potent transcription activation domain located in the C terminus of mouse p105. In addition, the I kappa B beta proteins chicken p40 and human MAD-3, proteins that are related to the p105 C terminus, strongly activated transcription in chicken cells and yeast when fused to GAL4 DNA-binding sequences. Furthermore, chicken p40 is primarily located in the nucleus of chicken cells when overexpressed from a retroviral vector. Our results suggest novel models for the function and regulation of NF-kappa B transcription complexes.

MeSH Terms
Amino Acid Sequence Animals Chickens DNA-Binding Proteins/chemistry,genetics Fluorescent Antibody Technique Fungal Proteins/genetics Gene Expression Regulation/genetics Humans I-kappa B Proteins Mice Molecular Sequence Data NF-KappaB Inhibitor alpha NF-kappa B/antagonists & inhibitors,chemistry,genetics NF-kappa B p50 Subunit Plasmids/genetics Protein Precursors/chemistry,genetics Recombinant Fusion Proteins/genetics Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors/genetics Transcription, Genetic/genetics Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae I kappa B beta protein I-kappa B Proteins NF-kappa B NF-kappa B p50 Subunit NFKBIA protein, human Nfkbia protein, mouse Protein Precursors Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors NF-KappaB Inhibitor alpha
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morin P J
Department of Biology, Boston University, MA 02215.
Gilmore T D
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.10.2453
Published
1992-05-25
Pages
2453-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312378
Subset
IM
Grants
NCI NIH HHS · CA47763 · United States
NCRR NIH HHS · SO7RR07043-26 · United States
External Links
PubMed source DOI: 10.1093/nar/20.10.2453 PMC Full Text View journal details
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