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PMID: 16000371 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.

Molecular biology of the cell ·Vol. 16 ·No. 9 ·2005-09-00 ·Pages 4350-61

Huot ME, Bisson N, Davidovic L, Mazroui R, Labelle Y, Moss T, Khandjian EW

Abstract

Fragile X-related 1 protein (FXR1P) is a member of a small family of RNA-binding proteins that includes the Fragile X mental retardation 1 protein (FMR1P) and the Fragile X-related 2 protein (FXR2P). These proteins are thought to transport mRNA and to control their translation. While FMR1P is highly expressed in neurons, substantial levels of FXR1P are found in striated muscles and heart, which are devoid of FMRP and FXR2P. However, little is known about the functions of FXR1P. We have isolated cDNAs for Xenopus Fxr1 and found that two specific splice variants are conserved in evolution. Knockdown of xFxr1p in Xenopus had highly muscle-specific effects, normal MyoD expression being disrupted, somitic myotomal cell rotation and segmentation being inhibited, and dermatome formation being abnormal. Consistent with the absence of the long muscle-specific xFxr1p isoform during early somite formation, these effects could be rescued by both the long and short mRNA variants. Microarray analyses showed that xFxr1p depletion affected the expression of 129 known genes of which 50% were implicated in muscle and nervous system formation. These studies shed significant new light on Fxr1p function(s).

MeSH Terms
3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Blastomeres/metabolism Cleavage Stage, Ovum/metabolism Embryo, Nonmammalian/metabolism Fragile X Mental Retardation Protein/metabolism,physiology Genetic Variation Humans Mice Molecular Sequence Data Muscle, Skeletal/embryology MyoD Protein/biosynthesis,genetics RNA, Messenger/antagonists & inhibitors,genetics,metabolism RNA-Binding Proteins/metabolism,physiology Sequence Alignment Somites/metabolism,physiology Xenopus Proteins/metabolism,physiology
Chemicals
3' Untranslated Regions FXR1 protein, Xenopus Fmr1 protein, Xenopus MyoD Protein RNA, Messenger RNA-Binding Proteins Xenopus Proteins Fragile X Mental Retardation Protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Huot Marc-Etienne
Unité de recherche en génétique humaine et moléculaire, CHUQ-St-François d'Assise, Québec, Québec G1L 3L5, Canada.
Bisson Nicolas
Davidovic Laetitia
Mazroui Rachid
Labelle Yves
Moss Tom
Khandjian Edouard W
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-04-0304
Published
2005-09-00
Epub
2005-00-06
Pages
4350-61
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1196343
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e05-04-0304 PMC Full Text View journal details
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