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PMID: 2426570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

UV crosslinking of RNA to nylon membrane enhances hybridization signals.

Molecular biology reports ·Vol. 11 ·No. 2 ·1986-00-00 ·Pages 107-15

Khandjian EW

Abstract

An improvement in the detection by nucleic acid hybridization of size-fractionated RNA immobilized to nylon-based membranes is described. Electrophoretic transfer of RNA to nylon membranes permits a quantitative determination of different RNA transcripts on the same membrane after sequential hybridization using different 32P-labeled DNA probes. UV crosslinking of the RNA to the nylon membrane increased the intensity of the radioactive signals. Using the method reported here, increased signals of between 10 and 40 fold were observed, depending on the species of transcript tested. Moderately abundant as well as rare transcripts can easily be detected in as little as 5 micrograms total cellular RNA.

MeSH Terms
Animals Cells, Cultured Kidney Membranes, Artificial Mice Nucleic Acid Hybridization/radiation effects Nylons RNA/biosynthesis,metabolism,radiation effects Ultraviolet Rays
Chemicals
Membranes, Artificial Nylons RNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Khandjian E W
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24 references, click to expand
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00364822
Published
1986-00-00
Pages
107-15
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00364822 View journal details
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