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PMID: 1620121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Growth-regulated expression of rhoG, a new member of the ras homolog gene family.

Molecular and cellular biology ·Vol. 12 ·No. 7 ·1992-07-00 ·Pages 3138-48

Vincent S, Jeanteur P, Fort P

Abstract

Cellular transition from the resting state to DNA synthesis involves master switches genes encoding transcriptional factors (e.g., fos, jun, and egr genes), whose targets remain to be fully characterized. To isolate coding sequences specifically accumulated in late G1, a differential screening was performed on a cDNA library prepared from hamster lung fibroblasts stimulated for 5 h with serum. One of the positive clones which displayed a sevenfold induction, turned out to code for a protein sharing homology to Ras-like products. Cloning and sequence analysis of the human homolog revealed that this putative new small GTPase, referred to as rhoG, is more closely related to the rac, CDC42, and TC10 members of the rho (ras homolog) gene family and might have diverged very early during evolution. rhoG mRNA accumulates in proportion to the mitogenic strength of various purified growth factors used for the stimulation, as a consequence of transcriptional activation. G1-specific RNA accumulation is impaired upon addition of antimitogenic cyclic AMP and is enhanced when protein synthesis is inhibited, mainly as a result of RNA stabilization. rhoG mRNA expression is observed in a wide variety of human organs but reaches a particularly high level in lung and placental tissues.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cricetinae Cycloheximide/pharmacology Enzyme Induction Fibroblasts GTP Phosphohydrolases/genetics Gene Expression Regulation Gene Library Genes, ras/genetics Humans Lung Molecular Sequence Data Multigene Family/genetics Phylogeny RNA, Messenger/biosynthesis Sequence Homology, Nucleic Acid Tissue Distribution Transcription Factors/biosynthesis Transcription, Genetic/drug effects rho GTP-Binding Proteins
Chemicals
RNA, Messenger Transcription Factors RHOG protein, human Cycloheximide GTP Phosphohydrolases rho GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vincent S
URA CNRS 1191 Génétique Moléculaire, Université Montpellier II Sciences et Techniques du Languedoc, France.
Jeanteur P
Fort P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.7.3138-3148.1992
Published
1992-07-00
Pages
3138-48
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364528
Subset
IM
Databases
GENBANK
X61587, X61588
External Links
PubMed source DOI: 10.1128/mcb.12.7.3138-3148.1992 PMC Full Text View journal details
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