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PMID: 16219790 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Growth defect and mutator phenotypes of RecQ-deficient Neurospora crassa mutants separately result from homologous recombination and nonhomologous end joining during repair of DNA double-strand breaks.

Genetics ·Vol. 172 ·No. 1 ·2006-01-00 ·Pages 113-25

Kato A, Inoue H

Abstract

RecQ helicases function in the maintenance of genome stability in many organisms. The filamentous fungus Neurospora crassa has two RecQ homologs, QDE3 and RECQ2. We found that the qde-3 recQ2 double mutant showed a severe growth defect. The growth defect was alleviated by mutation in mei-3, the homolog of yeast RAD51, which is required for homologous recombination (HR), suggesting that HR is responsible for this phenotype. We also found that the qde-3 recQ2 double mutant showed a mutator phenotype, yielding mostly deletions. This phenotype was completely suppressed by mutation of mus-52, a homolog of the human KU80 gene that is required for nonhomologous end joining (NHEJ), but was unaffected by mutation of mei-3. The high spontaneous mutation frequency in the double mutant is thus likely to be due to NHEJ acting on an elevated frequency of double-strand breaks (DSBs) and we therefore suggest that QDE3 and RECQ2 maintain chromosome stability by suppressing the formation of spontaneous DSBs.

MeSH Terms
Adenosine Triphosphatases/deficiency,genetics Antigens, Nuclear/genetics Chromosome Breakage DNA/genetics,metabolism DNA Helicases/deficiency,genetics,metabolism DNA Repair DNA, Fungal/genetics,metabolism DNA-Binding Proteins/genetics Epistasis, Genetic Fungal Proteins/genetics,metabolism Gene Deletion Ku Autoantigen Mutation/genetics Neurospora crassa/enzymology,genetics,growth & development Phenotype RecQ Helicases Recombination, Genetic Suppression, Genetic
Chemicals
Antigens, Nuclear DNA, Fungal DNA-Binding Proteins Fungal Proteins MEI-3 protein, Neurospora crassa DNA Adenosine Triphosphatases RECQL protein, human DNA Helicases RecQ Helicases Xrcc6 protein, human Ku Autoantigen QDE-3 protein, Neurospora crassa
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kato Akihiro
Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University, 338-8570 Saitama, Japan.
Inoue Hirokazu
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.041756
Published
2006-01-00
Epub
2005-00-11
Pages
113-25
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456140
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.105.041756 PMC Full Text View journal details
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