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PMID: 16330711 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Dendritic BC1 RNA in translational control mechanisms.

The Journal of cell biology ·Vol. 171 ·No. 5 ·2005-12-05 ·Pages 811-21

Wang H, Iacoangeli A, Lin D, Williams K, Denman RB, Hellen CU, Tiedge H

Abstract

Translational control at the synapse is thought to be a key determinant of neuronal plasticity. How is such control implemented? We report that small untranslated BC1 RNA is a specific effector of translational control both in vitro and in vivo. BC1 RNA, expressed in neurons and germ cells, inhibits a rate-limiting step in the assembly of translation initiation complexes. A translational repression element is contained within the unique 3' domain of BC1 RNA. Interactions of this domain with eukaryotic initiation factor 4A and poly(A) binding protein mediate repression, indicating that the 3' BC1 domain targets a functional interaction between these factors. In contrast, interactions of BC1 RNA with the fragile X mental retardation protein could not be documented. Thus, BC1 RNA modulates translation-dependent processes in neurons and germs cells by directly interacting with translation initiation factors.

MeSH Terms
3' Flanking Region Animals Dendrites/metabolism Eukaryotic Initiation Factor-4A/genetics,metabolism Female Fragile X Mental Retardation Protein/genetics,metabolism In Vitro Techniques Oocytes/metabolism Poly(A)-Binding Proteins/genetics,metabolism Protein Binding Protein Biosynthesis RNA Transport RNA, Messenger/metabolism RNA, Small Cytoplasmic/genetics,physiology Ribosomes/genetics,metabolism Xenopus laevis
Chemicals
BC1 RNA Poly(A)-Binding Proteins RNA, Messenger RNA, Small Cytoplasmic Fragile X Mental Retardation Protein Eukaryotic Initiation Factor-4A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Huidong
Department of Physiology and Pharmacology, State University of New York, Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.
Iacoangeli Anna
Lin Daisy
Williams Keith
Denman Robert B
Hellen Christopher U T
Tiedge Henri
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200506006
Published
2005-12-05
Pages
811-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC1828541
Subset
IM
Grants
NIAID NIH HHS · R01 AI051340 · United States
NINDS NIH HHS · R01 NS046769 · United States
NIAID NIH HHS · AI51340 · United States
NINDS NIH HHS · NS046769 · United States
NIAID NIH HHS · R56 AI051340 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1083/jcb.200506006 PMC Full Text View journal details
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