Home LiteratureArticle Details
PMID: 16339080 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans.

Molecular biology of the cell ·Vol. 17 ·No. 2 ·2006-02-00 ·Pages 1018-32

Enjalbert B, Smith DA, Cornell MJ, Alam I, Nicholls S, Brown AJ, Quinn J

Abstract

The resistance of Candida albicans to many stresses is dependent on the stress-activated protein kinase (SAPK) Hog1. Hence we have explored the role of Hog1 in the regulation of transcriptional responses to stress. DNA microarrays were used to characterize the global transcriptional responses of HOG1 and hog1 cells to three stress conditions that activate the Hog1 SAPK: osmotic stress, oxidative stress, and heavy metal stress. This revealed both stress-specific transcriptional responses and a core transcriptional response to stress in C. albicans. The core transcriptional response was characterized by a subset of genes that responded in a stereotypical manner to all of the stresses analyzed. Inactivation of HOG1 significantly attenuated transcriptional responses to osmotic and heavy metal stresses, but not to oxidative stress, and this was reflected in the role of Hog1 in the regulation of C. albicans core stress genes. Instead, the Cap1 transcription factor plays a key role in the oxidative stress regulation of C. albicans core stress genes. Our data show that the SAPK network in C. albicans has diverged from corresponding networks in model yeasts and that the C. albicans SAPK pathway functions in parallel with other pathways to regulate the core transcriptional response to stress.

MeSH Terms
Basic-Leucine Zipper Transcription Factors Candida albicans/enzymology,genetics Cell Cycle Proteins/metabolism Cluster Analysis Fungal Proteins/genetics,metabolism,physiology Gene Deletion Gene Expression Profiling Gene Expression Regulation, Fungal Genome, Fungal Metals, Heavy/metabolism Mitogen-Activated Protein Kinases/genetics,physiology Models, Biological Osmotic Pressure Oxidative Stress RNA, Messenger/metabolism Saccharomycetales/metabolism Schizosaccharomyces/metabolism Signal Transduction
Chemicals
Basic-Leucine Zipper Transcription Factors CAP1 protein, Candida albicans Cell Cycle Proteins Fungal Proteins Metals, Heavy RNA, Messenger Mitogen-Activated Protein Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Enjalbert Brice
Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom.
Smith Deborah A
Cornell Michael J
Alam Intikhab
Nicholls Susan
Brown Alistair J P
Quinn Janet
References (48)
48 references, click to expand
  1. Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.
    Mol Biol Cell. 2002 Mar;13(3):805-16 PMID: 11907263
  2. Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium.
    Environ Toxicol Chem. 2001 Oct;20(10):2353-60 PMID: 11596770
  3. The serine/threonine kinase Cmk2 is required for oxidative stress response in fission yeast.
    J Biol Chem. 2002 May 17;277(20):17722-7 PMID: 11886858
  4. Osmotic stress signaling and osmoadaptation in yeasts.
    Microbiol Mol Biol Rev. 2002 Jun;66(2):300-72 PMID: 12040128
  5. The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast.
    J Biol Chem. 2002 Sep 6;277(36):33411-21 PMID: 12080074
  6. Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence.
    Microbiology. 2002 Nov;148(Pt 11):3705-13 PMID: 12427960
  7. Global transcriptional responses of fission yeast to environmental stress.
    Mol Biol Cell. 2003 Jan;14(1):214-29 PMID: 12529438
  8. The Hog1 mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans.
    Eukaryot Cell. 2003 Apr;2(2):351-61 PMID: 12684384
  9. Stress-induced gene expression in Candida albicans: absence of a general stress response.
    Mol Biol Cell. 2003 Apr;14(4):1460-7 PMID: 12686601
  10. Candida albicans response regulator gene SSK1 regulates a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress.
    Eukaryot Cell. 2003 Oct;2(5):1018-24 PMID: 14555484
  11. Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis.
    Mol Biol Cell. 2004 Feb;15(2):532-42 PMID: 14595107
  12. The Schizosaccharomyces pombe HIRA-like protein Hip1 is required for the periodic expression of histone genes and contributes to the function of complex centromeres.
    Mol Cell Biol. 2004 May;24(10):4309-20 PMID: 15121850
  13. Activation of the redox sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration.
    Mol Microbiol. 2004 Jun;52(5):1427-35 PMID: 15165244
  14. Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae.
    Proteomics. 2004 Aug;4(8):2425-36 PMID: 15274137
  15. A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans.
    Mol Biol Cell. 2004 Sep;15(9):4179-90 PMID: 15229284
  16. Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1.
    Nat Cell Biol. 2004 Oct;6(10):997-1002 PMID: 15448699
  17. Msn2- and Msn4-like transcription factors play no obvious roles in the stress responses of the fungal pathogen Candida albicans.
    Eukaryot Cell. 2004 Oct;3(5):1111-23 PMID: 15470239
  18. Oxygen toxicity, oxygen radicals, transition metals and disease.
    Biochem J. 1984 Apr 1;219(1):1-14 PMID: 6326753
  19. Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins.
    Genes Dev. 1989 Mar;3(3):283-92 PMID: 2542125
  20. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  21. Getting started with yeast.
    Methods Enzymol. 1991;194:3-21 PMID: 2005794
  22. Isogenic strain construction and gene mapping in Candida albicans.
    Genetics. 1993 Jul;134(3):717-28 PMID: 8349105
  23. Oxidative mechanisms in the toxicity of metal ions.
    Free Radic Biol Med. 1995 Feb;18(2):321-36 PMID: 7744317
  24. Analysis of the adaptive oxidative stress response of Candida albicans.
    FEMS Microbiol Lett. 1996 Apr 15;138(1):83-8 PMID: 8674975
  25. The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans.
    J Bacteriol. 1996 Oct;178(19):5850-2 PMID: 8824643
  26. Nonfilamentous C. albicans mutants are avirulent.
    Cell. 1997 Sep 5;90(5):939-49 PMID: 9298905
  27. Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.
    Mol Cell Biol. 1997 Dec;17(12):6982-93 PMID: 9372930
  28. Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene.
    Infect Immun. 1998 May;66(5):1953-61 PMID: 9573075
  29. Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.
    Genes Dev. 1998 May 15;12(10):1453-63 PMID: 9585505
  30. Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae.
    Yeast. 1998 Sep 30;14(13):1209-21 PMID: 9791892
  31. Stress-activated signalling pathways in yeast.
    Genes Cells. 1998 Aug;3(8):485-98 PMID: 9797451
  32. The bZip transcription factor Cap1p is involved in multidrug resistance and oxidative stress response in Candida albicans.
    J Bacteriol. 1999 Feb;181(3):700-8 PMID: 9922230
  33. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.
    J Bacteriol. 1999 Mar;181(6):1868-74 PMID: 10074081
  34. Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans.
    J Bacteriol. 1999 May;181(10):3058-68 PMID: 10322006
  35. CandidaDB: a genome database for Candida albicans pathogenomics.
    Nucleic Acids Res. 2005 Jan 1;33(Database issue):D353-7 PMID: 15608215
  36. Global roles of Ssn6 in Tup1- and Nrg1-dependent gene regulation in the fungal pathogen, Candida albicans.
    Mol Biol Cell. 2005 Jun;16(6):2913-25 PMID: 15814841
  37. Oxidation of a eukaryotic 2-Cys peroxiredoxin is a molecular switch controlling the transcriptional response to increasing levels of hydrogen peroxide.
    J Biol Chem. 2005 Jun 17;280(24):23319-27 PMID: 15824112
  38. Gene disruption in Candida albicans using a synthetic, codon-optimised Cre-loxP system.
    Fungal Genet Biol. 2005 Sep;42(9):737-48 PMID: 16043373
  39. Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1.
    Mol Cell Biol. 2000 Jun;20(11):3887-95 PMID: 10805732
  40. Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p.
    Mol Microbiol. 2000 May;36(3):618-29 PMID: 10844651
  41. Genomic expression programs in the response of yeast cells to environmental changes.
    Mol Biol Cell. 2000 Dec;11(12):4241-57 PMID: 11102521
  42. Remodeling of yeast genome expression in response to environmental changes.
    Mol Biol Cell. 2001 Feb;12(2):323-37 PMID: 11179418
  43. Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.
    EMBO J. 2001 Mar 1;20(5):1123-33 PMID: 11230135
  44. Significance analysis of microarrays applied to the ionizing radiation response.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21 PMID: 11309499
  45. Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast.
    Proc Natl Acad Sci U S A. 2001 May 8;98(10):5625-30 PMID: 11344302
  46. A proteome analysis of the cadmium response in Saccharomyces cerevisiae.
    J Biol Chem. 2001 Mar 16;276(11):8469-74 PMID: 11078740
  47. Cassettes for PCR-mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicans.
    Yeast. 2001 Jun 30;18(9):859-64 PMID: 11427968
  48. Sulfur sparing in the yeast proteome in response to sulfur demand.
    Mol Cell. 2002 Apr;9(4):713-23 PMID: 11983164
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-06-0501
Published
2006-02-00
Epub
2005-00-07
Pages
1018-32
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1356608
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/C510383/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/C510391/1 · United Kingdom
External Links
PubMed source DOI: 10.1091/mbc.e05-06-0501 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2