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PMID: 16382141 Published · ppublish English Journal Article

H2A.Z functions to regulate progression through the cell cycle.

Molecular and cellular biology ·Vol. 26 ·No. 2 ·2006-01-00 ·Pages 489-501

Dhillon N, Oki M, Szyjka SJ, Aparicio OM, Kamakaka RT

Abstract

Histone H2A variants are highly conserved proteins found ubiquitously in nature and thought to perform specialized functions in the cell. Studies in yeast on the histone H2A variant H2A.Z have shown a role for this protein in transcription as well as chromosome segregation. Our studies have focused on understanding the role of H2A.Z during cell cycle progression. We found that htz1delta cells were delayed in DNA replication and progression through the cell cycle. Furthermore, cells lacking H2A.Z required the S-phase checkpoint pathway for survival. We also found that H2A.Z localized to the promoters of cyclin genes, and cells lacking H2A.Z were delayed in the induction of these cyclin genes. Several different models are proposed to explain these observations.

MeSH Terms
Cell Cycle/physiology Cyclin B/genetics,metabolism Cyclins/genetics,metabolism DNA Replication Histones/genetics,metabolism Mutation Promoter Regions, Genetic Replication Origin S Phase/physiology Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
CLB5 protein, S cerevisiae CLN2 protein, S cerevisiae Cyclin B Cyclins Histones Htz1 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dhillon Namrita
Unit on Chromatin and Transcription, National Institute of Child Health and Human Development, National Institutes of Health, Bldg. 18T, Rm. 106, 18 Library Dr., Bethesda, Maryland 20892, USA.
Oki Masaya
Szyjka Shawn J
Aparicio Oscar M
Kamakaka Rohinton T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.26.2.489-501.2006
Published
2006-01-00
Pages
489-501
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1346916
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.26.2.489-501.2006 PMC Full Text View journal details
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