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PMID: 16397796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments.

Planta ·Vol. 224 ·No. 2 ·2006-07-00 ·Pages 300-14

Teixeira FK, Menezes-Benavente L, Galvão VC, Margis R, Margis-Pinheiro M

Abstract

Aerobic organisms evolved a complex antioxidant system, which protect the cells against oxidative damage caused by partially reduced oxygen intermediates, also known as reactive oxygen species. In plants, ascorbate peroxidases (EC, 1.11.1.11) catalyze the conversion of H(2)O(2) to H(2)O, using ascorbate as the specific electron donor in this enzymatic reaction. Previously, eight APx genes were identified in the rice (Oryza sativa L.) genome through in silico analysis: two cytosolic isoforms, two putative peroxisomal isoforms, and four putative chloroplastic ones. Using gene-specific probes, we confirmed the presence of the eight APx genes in the rice genome by Southern blot hybridization. Transcript accumulation analysis showed specific expression patterns for each member of the APx family according to developmental stage and in response to salt stress, revealing the complexity of the antioxidant system in plants. Finally, the subcellular localization of rice APx isoforms was determined using GFP-fusion proteins in BY-2 tobacco cells. In agreement with the initial prediction, OSAPX3 was localized in the peroxisomes. On the other hand, the OSAPX6-GFP fusion protein was found in mitochondria of the BY-2 cells, in contrast to the chloroplastic location predicted by sequence analysis. Our findings reveal the functional diversity of the rice APx genes and suggest complementation and coordination of the antioxidant defenses in different cellular compartments during development and abiotic stress.

MeSH Terms
Ascorbate Peroxidases Blotting, Northern Blotting, Southern Flowers/metabolism Gene Expression Regulation, Plant/drug effects Isoenzymes/metabolism Oryza/drug effects,enzymology Peroxidases/genetics,metabolism Plant Leaves/metabolism Plant Stems/metabolism Plants, Genetically Modified Protein Transport RNA, Messenger/genetics,metabolism Recombinant Fusion Proteins/metabolism Reverse Transcriptase Polymerase Chain Reaction Salts/pharmacology Seedlings/metabolism Subcellular Fractions/enzymology Tobacco/cytology
Chemicals
Isoenzymes RNA, Messenger Recombinant Fusion Proteins Salts Peroxidases Ascorbate Peroxidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Teixeira Felipe Karam
Laboratório de Genética Molecular Vegetal, Departamento de Genética, Universidade Federal do Rio de Janeiro, 21944-970, Rio de Janeiro, Brasil.
Menezes-Benavente Larissa
Galvão Vinícius Costa
Margis Rogério
Margis-Pinheiro Márcia
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-005-0214-8
Published
2006-07-00
Epub
2006-00-06
Pages
300-14
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-005-0214-8 View journal details
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