Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.

Current genetics ·Vol. 19 ·No. 1 ·1991-01-00 ·Pages 9-14

Oulmouden A, Karst F

Abstract

The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with the yeast gene RAR1, isolated elsewhere by complementation of a rar1 mutation involved in the stability of plasmids with weak ARS. In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast.

Related Genes

ERG12 RAR1

MeSH Terms

Amino Acid Sequence Base Sequence Ergosterol/biosynthesis Genes, Fungal Molecular Sequence Data Phosphotransferases/antagonists & inhibitors,genetics,metabolism Phosphotransferases (Alcohol Group Acceptor) Polyisoprenyl Phosphates/pharmacology Promoter Regions, Genetic Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sesquiterpenes

Chemicals

Polyisoprenyl Phosphates Sesquiterpenes geranyl pyrophosphate farnesyl pyrophosphate Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) mevalonate kinase Ergosterol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Oulmouden A

Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.

Karst F

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25 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00362081

Published

1991-01-00

Pages

9-14

Language

English

Region

United States

NLM ID

8004904

Subset

Databases

GENBANK

S39723, S39727, S39777, S60919, S60920, X55875, X58894, X62724, X62725, X62800

External Links

PubMed source DOI: 10.1007/BF00362081 View journal details

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Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.

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