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PMID: 16464828 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray.

Nucleic acids research ·Vol. 34 ·No. 3 ·2006-00-00 ·Pages 986-95

Graindorge A, Thuret R, Pollet N, Osborne HB, Audic Y

Abstract

Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray analysis to identify mRNAs that are regulated by changes in their adenylation state during oogenesis and early development of the diploid frog Xenopus tropicalis. The microarray data were validated using qRT-PCR and direct analysis of the adenylation state of endogenous maternal mRNAs during the period studied. We identified more than 500 mRNAs regulated at the post-transcriptional level among the 3000 mRNAs potentially detected by the microarray. The mRNAs were classified into nine different adenylation behavior categories. The various adenylation profiles observed during oocyte maturation and early development and the analyses of 3'-untranslated region sequences suggest that previously uncharacterized sequence elements control the adenylation behavior of the newly identified mRNAs. These data should prove useful in identifying mRNAs with important functions during oocyte maturation and early development.

MeSH Terms
3' Untranslated Regions/chemistry Animals Cells, Cultured Embryo, Nonmammalian/metabolism Female Gene Expression Regulation, Developmental Meiosis Oligonucleotide Array Sequence Analysis Oocytes/cytology,metabolism Oogenesis Polyadenylation RNA 3' Polyadenylation Signals RNA, Messenger/metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Xenopus
Chemicals
3' Untranslated Regions RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Graindorge Antoine
CNRS UMR 6061, IFR 140, Functional Genetics, Agricultural and Health Sciences, Faculté de Médecine, Université de Rennes, 1 Rennes, France.
Thuret Raphaël
Pollet Nicolas
Osborne H Beverley
Audic Yann
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkj492
Published
2006-00-00
Epub
2006-00-07
Pages
986-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1361620
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkj492 PMC Full Text View journal details
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