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PMID: 1648227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of a fos-lacZ fusion gene: a paradigm for quantitative analysis of stimulus-transcription coupling.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 13 ·1991-07-01 ·Pages 5665-9

Schilling K, Luk D, Morgan JI, Curran T

Abstract

Expression of the c-fos protooncogene is induced by a great variety of extracellular stimuli. A fos-lacZ fusion gene has been constructed that recapitulates this regulation. The fos-lacZ gene was introduced into B104 neuroblastoma cells for use in a quantitative assay for stimulus-transcription coupling. Both alpha- and beta-adrenergic agonists, dibutyryl cAMP, and phorbol ester induced beta-galactosidase activity in a dose-dependent manner. Thus, the interactions of receptors with agonists and antagonists, as well as intracellular second messenger-mediated signaling events, can be analyzed quantitatively. This approach represents a prototypic method for investigating stimulus-response coupling based upon gene expression.

MeSH Terms
Animals Bucladesine/pharmacology Cells, Cultured Dose-Response Relationship, Drug Gene Expression Regulation In Vitro Techniques Isoproterenol/pharmacology Mice Norepinephrine/analogs & derivatives,pharmacology Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Recombinant Fusion Proteins/genetics Regulatory Sequences, Nucleic Acid Restriction Mapping Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic Transfection beta-Galactosidase/genetics
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Recombinant Fusion Proteins Bucladesine 6-fluoronorepinephrine beta-Galactosidase Isoproterenol Tetradecanoylphorbol Acetate Norepinephrine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schilling K
Department of Neurosciences, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Luk D
Morgan J I
Curran T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.13.5665
Published
1991-07-01
Pages
5665-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51938
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.13.5665 PMC Full Text View journal details
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