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PMID: 2498083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 805-13

Zerial M, Toschi L, Ryseck RP, Schuermann M, Müller R, Bravo R

Abstract

We have identified a gene, fos B, encoding a nuclear protein of 338 amino acids presenting a 70% homology with c-fos, whose expression is activated during G0/G1 transition. Growth factor stimulation of quiescent cells leads to a rapid and transient accumulation of fos B mRNA, with kinetics similar to those of c-fos. The induction of fos B mRNA levels is in part due to a dramatic increase in the transcription of the gene. The half-life of fos B mRNA is in the order of 10-15 min. Both transcriptional activation and mRNA stability are substantially increased in the presence of protein synthesis inhibitors. Immunoprecipitation studies showed that fos B as c-fos protein, forms a complex in vitro with c-jun and jun B proteins in the absence of a target binding sequence. Gel retardation assays demonstrated that fos B protein positively influences the binding of c-jun and jun B proteins to an AP-1 binding consensus sequence, suggesting that fos B protein plays a role in control of gene expression.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites DNA/genetics DNA-Binding Proteins/metabolism Gene Expression Regulation Mice Molecular Sequence Data Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Proto-Oncogenes Sequence Homology, Nucleic Acid Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zerial M
European Molecular Biology Laboratory, Heidelberg, FRG.
Toschi L
Ryseck R P
Schuermann M
Müller R
Bravo R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
805-13
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400877
Subset
IM
Databases
GENBANK
X14897
External Links
PubMed source PMC Full Text View journal details
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