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PMID: 165495 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Melanoma cells resistant to inhibition of growth by melanocyte stimulating hormone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 951-5

Pawelek J, Sansone M, Koch N, Christie G, Halaban R, Hendee J, Lerner AB, Varga JM

Abstract

Melanocyte stimulating hormone (MSH) enhances melanization but inhibits proliferation of Cloudman S91 melanoma cells in culture. We have isolated variants of these cells that can grow in the presence of MSH. The conclusions we have reached from analyses of these cells are the following: (1) Basal tyrosinase activity (monophenol monooxygenase; monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, EC 1.14.18.1), i.e., the activity that is present in the absence of added MSH, is related through a common biochemical pathway to MSH-mediated control of growth. (2) MSH-inducible tyrosinase activity does not appear to be related to MSH control of growth. (3) The morphological changes that occur following the addition of MSH or cAMP are related to controls of growth and not to those of melanization.

MeSH Terms
Animals Catechol Oxidase/metabolism Cell Division/drug effects Cell Line Cyclic AMP/metabolism DNA, Neoplasm/metabolism Melanocyte-Stimulating Hormones/metabolism,pharmacology Melanoma/enzymology,metabolism Mice Neoplasms, Experimental Phenotype Protein Kinases/metabolism Receptors, Cell Surface
Chemicals
DNA, Neoplasm Receptors, Cell Surface Melanocyte-Stimulating Hormones Cyclic AMP Catechol Oxidase Protein Kinases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pawelek J
Sansone M
Koch N
Christie G
Halaban R
Hendee J
Lerner A B
Varga J M
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.951
Published
1975-03-00
Pages
951-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432441
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.951 PMC Full Text View journal details
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