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PMID: 1655697 Published · ppublish English Comparative Study Journal Article

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

Journal of bacteriology ·Vol. 173 ·No. 19 ·1991-10-00 ·Pages 6074-87

Crouzet J, Levy-Schil S, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Debussche L, Thibaut D

Abstract

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named cobN to cobQ and cobW. Based on the similarity of NH2-terminal sequences and molecular weights of the purified Cob proteins, CobQ was identified as cobyric acid synthase, CobP was identified as a bifunctional enzyme exhibiting both cobinamide kinase and cobinamide phosphate guanylyltransferase activities, and CobO was identified as cob(I)alamin adenosyltransferase. CobN is proposed to play a role in cobalt insertion reactions. Four other open reading frames were identified on the 13.1-kb fragment, but their chromosomal inactivation did not lead to a cobalamin-minus phenotype.

Related Genes
cob cobN cobQ cobW
MeSH Terms
Alkyl and Aryl Transferases Amino Acid Sequence Base Composition Base Sequence DNA, Bacterial/chemistry Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Molecular Weight Multienzyme Complexes/chemistry,genetics Mutagenesis, Insertional Nucleotidyltransferases/biosynthesis,genetics Phosphotransferases/biosynthesis,genetics Phosphotransferases (Alcohol Group Acceptor) Pseudomonas/genetics Transaminases/biosynthesis,genetics Transferases/genetics Vitamin B 12/biosynthesis,chemistry,genetics
Chemicals
DNA, Bacterial Multienzyme Complexes Transferases Alkyl and Aryl Transferases cob(I)alamin adenosyltransferase Transaminases cobyric acid synthase Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) cobinamide kinase Nucleotidyltransferases cobinamide phosphate guanylyltransferase Vitamin B 12
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Crouzet J
Unité de Biologie Moléculaire, Institut des Biotechnologies, Vitry-sur-Seine, France.
Levy-Schil S
Cameron B
Cauchois L
Rigault S
Rouyez M C
Blanche F
Debussche L
Thibaut D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.19.6074-6087.1991
Published
1991-10-00
Pages
6074-87
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208354
Subset
IM
Databases
GENBANK
M62866, M64126, M64127, M64128, M64129, S52988, S52991, S53821, S56866, X61007
External Links
PubMed source DOI: 10.1128/jb.173.19.6074-6087.1991 PMC Full Text View journal details
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