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PMID: 1657889 Published · ppublish English Journal Article

Gene localization, size, and physical map of the chromosome of Streptococcus pneumoniae.

Journal of bacteriology ·Vol. 173 ·No. 22 ·1991-11-00 ·Pages 7361-7

Gasc AM, Kauc L, Barraillé P, Sicard M, Goodgal S

Abstract

A physical map of the Streptococcus (Diplococcus) pneumoniae chromosome, which is circular and 2,270 kbp in circumference, has been constructed. The restriction enzymes ApaI, SmaI, and SacII were used to digest intact chromosomes, and the fragments were resolved by field inversion gel electrophoresis (FIGE). The digests produced 22, 20, and 29 fragments, respectively. The order of the fragments was deduced from Southern blot hybridization of isolated labeled fragments to separated fragments of the various restriction digests. Genetic markers were correlated with the physical map by transformation of recipient cells with FIGE-isolated DNA fragments derived from genetically marked S. pneumoniae strains. In addition, markers were mapped by the hybridization of cloned genes to FIGE-separated restriction fragments. Six rRNA gene (rrn) clusters were mapped by hybridization to rrn-containing fragments of Haemophilus influenzae.

MeSH Terms
Base Sequence Chromosomes, Bacterial DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics,isolation & purification DNA, Circular/genetics Deoxyribonucleases, Type II Site-Specific/metabolism Genetic Markers Restriction Mapping Streptococcus pneumoniae/genetics Substrate Specificity
Chemicals
DNA, Bacterial DNA, Circular Genetic Markers DNA Restriction Enzymes CCCGGG-specific type II deoxyribonucleases CCGCGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific GGGCCC-specific type II deoxyribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gasc A M
Centre de Recherche de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
Kauc L
Barraillé P
Sicard M
Goodgal S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.22.7361-7367.1991
Published
1991-11-00
Pages
7361-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209245
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.22.7361-7367.1991 PMC Full Text View journal details
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