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PMID: 2828317 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic transformation in Streptococcus pneumoniae: molecular cloning and characterization of recP, a gene required for genetic recombination.

Journal of bacteriology ·Vol. 170 ·No. 2 ·1988-02-00 ·Pages 630-7

Rhee DK, Morrison DA

Abstract

A 225-base-pair fragment of a recombination gene was identified by insertion-duplication mutagenesis and used as a radioactive probe to clone the corresponding rec locus from Streptococcus pneumoniae in Escherichia coli plasmid vectors. Attempts to clone large pieces of this locus were unsuccessful, but small pieces of DNA from this region were cloned in the E. coli transcriptional terminator vectors pKK232-8 and pJDC9. The extent of the rec region, 2.1 to 2.2 kilobases, was defined by determining the competence phenotype of insertion mutations constructed in vitro. A deletion of the rec locus showed it to be necessary for chromosomal integration but not for plasmid establishment. A plasmid carrying the entire locus encoded a 72-kilodalton polypeptide in a cell-free E. coli transcription-translation system.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genetic Vectors Mutation Nucleic Acid Hybridization Phenotype Plasmids Recombination, Genetic Streptococcus pneumoniae/genetics Transcription, Genetic Transformation, Bacterial
Chemicals
Bacterial Proteins DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rhee D K
Laboratory for Cell, Molecular, and Developmental Biology, University of Illinois at Chicago 60680.
Morrison D A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.2.630-637.1988
Published
1988-02-00
Pages
630-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210701
Subset
IM
Grants
NIAID NIH HHS · AI19875 · United States
External Links
PubMed source DOI: 10.1128/jb.170.2.630-637.1988 PMC Full Text View journal details
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