Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation.

Journal of bacteriology ·Vol. 138 ·No. 1 ·1979-04-00 ·Pages 80-6

Lefevre JC, Claverys JP, Sicard AM

Abstract

The efficiency of transformation of point mutations depends upon base pair mismatches during the recombination process. For low-efficiency markers, the genetic information carried on the donor deoxyribonucleic acid is preferentially lost. To understand this elimination process, we investigated the effect of the size of donor deoxyribonucleic acid on the relative efficiency of low-efficiency point mutations. The deoxyribonucleic acid was shortened either by mechanical shearing or by restriction enzyme treatments. The results indicate that transformation by low-efficiency markers was not affected by shortening the distance between them and the end of the molecule any more than was transformation by the other markers. Moreover, no lethal event could be detected for either cell or chromosomal marker survival. These data do not exclude the double-strand-break hypothesis that was proposed to explain the loss of genetic information for low-efficiency markers, but they offer no support for it.

MeSH Terms

DNA Restriction Enzymes/pharmacology DNA, Bacterial/genetics,metabolism Genes Mutation Streptococcus pneumoniae/genetics Transformation, Bacterial/drug effects

Chemicals

DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lefevre J C

Claverys J P

Sicard A M

References (26)

26 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.138.1.80-86.1979

Published

1979-04-00

Pages

80-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218240

Subset

External Links

PubMed source DOI: 10.1128/jb.138.1.80-86.1979 PMC Full Text View journal details

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Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation.

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