Home LiteratureArticle Details
PMID: 16593362 Published · ppublish English Journal Article

Variation at the 87A heat shock locus in Drosophila melanogaster.

Brown AJ

Abstract

Restriction maps for 25 kilobases of DNA around the 87A7 heat shock locus have been determined in 29 chromosomes isolated from a natural population. The heterozygosity per nucleotide and the proportion of polymorphic nucleotide sites were estimated to be 0.0024 and 0.007, respectively. The mean number of insertional differences in this region between random pairs of chromosomes was 0.95. A significant amount of this variation was due to the insertion of large transposable elements. All the insertion/deletion events were found in a region less than 2 kilobases in size. This could either be due to nonrandom integration or to differences in the intensity of selection against DNA insertion at different sites.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Brown A J
Imperial Cancer Research Fund, Mill Hill Laboratories, Burtonhole Lane, London NW7 1AD, United Kingdom.
References (55)
55 references, click to expand
  1. Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678-82 PMID: 6300868
  2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  3. The transposable element Mdg3 in Drosophila melanogaster is flanked with the perfect direct and mismatched inverted repeats.
    Nucleic Acids Res. 1980 Aug 11;8(15):3263-73 PMID: 6255410
  4. Polymorphic DNA region adjacent to the 5' end of the human insulin gene.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759-63 PMID: 6272317
  5. Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1091-5 PMID: 16592770
  6. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination.
    Genetics. 1977 Aug;86(4):813-33 PMID: 17248751
  7. Estimation of genetic variation at the DNA level from restriction endonuclease data.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3748-50 PMID: 6267603
  8. Physical mapping of the globin gene deletion in hereditary persistence of foetal haemoglobin (HPFH).
    Nucleic Acids Res. 1980 Apr 11;8(7):1521-34 PMID: 6159595
  9. Evolution of the beta-globin gene cluster in man and the primates.
    J Mol Biol. 1981 Jul 5;149(3):319-36 PMID: 6273584
  10. Highly variable regions of DNA flank the human alpha globin genes.
    Nucleic Acids Res. 1981 Sep 11;9(17):4213-24 PMID: 6272199
  11. Linkage analysis of nondeletion hereditary persistence of fetal hemoglobin.
    Science. 1982 Feb 19;215(4535):981-2 PMID: 6186021
  12. Evidence for multiple origins of the beta E-globin gene in Southeast Asia.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6608-11 PMID: 6292908
  13. Transposition of cloned P elements into Drosophila germ line chromosomes.
    Science. 1982 Oct 22;218(4570):341-7 PMID: 6289435
  14. General properties of mobile dispersed genetic elements in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:655-65 PMID: 6266757
  15. Hybrid dysgenesis in Drosophila and the Stochastic loss hypothesis.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:561-5 PMID: 6266749
  16. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family.
    Cell. 1982 Jul;29(3):995-1004 PMID: 6295641
  17. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations.
    Cell. 1982 Jul;29(3):987-94 PMID: 6295640
  18. Mobile dispersed genetic element MDG1 of Drosophila melanogaster: nucleotide sequence of long terminal repeats.
    Nucleic Acids Res. 1981 Jul 24;9(14):3451-64 PMID: 6269083
  19. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  20. Estimating genetic divergence and genetic variability with restriction endonucleases.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6329-33 PMID: 6273864
  21. Genomic organization and functional analysis of a deletion variant of the 87A7 heat shock locus of Drosophila melanogaster.
    J Mol Biol. 1982 Mar 5;155(3):267-80 PMID: 6281448
  22. DNA sequences flanking the starts of the hsp 70 and alpha beta heat shock genes are homologous.
    Dev Biol. 1981 Apr 30;83(2):291-300 PMID: 6263741
  23. Evolution of the 87A and 87C heat-shock loci in Drosophila.
    Nature. 1981 Apr 23;290(5808):677-82 PMID: 6261150
  24. Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes.
    Science. 1981 Sep 4;213(4512):1117-20 PMID: 6267694
  25. DNA polymorphism detectable by restriction endonucleases.
    Genetics. 1981 Jan;97(1):145-63 PMID: 6266912
  26. Intra- and interspecific variation of the mitochondrial genome in Rattus norvegicus and Rattus rattus: restriction enzyme analysis of variant mitochondrial DNA molecules and their evolutionary relationships.
    Genetics. 1981 Jan;97(1):125-43 PMID: 6266911
  27. Restriction map variation in the Adh region of Drosophila.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5631-5 PMID: 6291056
  28. Estimating genetic variability with restriction endonucleases.
    Genetics. 1982 Apr;100(4):711-9 PMID: 6288514
  29. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  30. Novel bacteriophage lambda cloning vector.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5172-6 PMID: 6254062
  31. Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3605-9 PMID: 6251473
  32. Two genes for the major heat-shock protein of Drosophila melanogaster arranged as an inverted repeat.
    Nucleic Acids Res. 1980 Jan 25;8(2):235-52 PMID: 6252541
  33. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
    Gene. 1980 Aug;10(3):249-59 PMID: 6254843
  34. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.
    Chromosoma. 1981;82(2):205-16 PMID: 6452995
  35. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
    Cell. 1982 Sep;30(2):517-28 PMID: 6814763
  36. Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster.
    Genetics. 1981 Aug;98(4):775-89 PMID: 6800878
  37. Evolutionary implications of a complex pattern of DNA sequence homology extending far upstream of the hsp70 genes at loci 87A7 and 87C1 in Drosophila melanogaster.
    J Mol Biol. 1982 Mar 25;156(1):21-35 PMID: 6808144
  38. Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes.
    Nucleic Acids Res. 1980 Aug 11;8(15):3439-57 PMID: 6777757
  39. A highly polymorphic locus in human DNA.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754-8 PMID: 6935681
  40. DNA sequence variants in the G gamma-, A gamma-, delta- and beta-globin genes of man.
    Cell. 1979 Sep;18(1):1-10 PMID: 509514
  41. Inter- and intraspecific variation in restriction maps of Drosophila mitochondrial DNAs.
    Nature. 1979 Oct 25;281(5733):696-9 PMID: 233134
  42. Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster.
    Cell. 1979 Dec;18(4):1351-8 PMID: 117906
  43. Organization of the multiple genes for the 70,000-dalton heat-shock protein in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5254-8 PMID: 116226
  44. Deletion mapping of two D. melanogaster loci that code for the 70,000 dalton heat-induced protein.
    Cell. 1979 Jul;17(3):565-71 PMID: 113104
  45. A new estimate of sequence divergence of mitochondrial DNA using restriction endonuclease mappings.
    J Mol Evol. 1979 Nov;13(4):295-304 PMID: 117114
  46. Genes for the 70,000 dalton heat shock protein in two cloned D. melanogaster DNA segments.
    Cell. 1979 May;17(1):9-18 PMID: 110461
  47. Physical map of two D. melanogaster DNA segments containing sequences coding for the 70,000 dalton heat shock protein.
    Cell. 1979 May;17(1):1-8 PMID: 110458
  48. Sequence organization of two recombinant plasmids containing genes for the major heat shock-induced protein of D. melanogaster.
    Cell. 1979 Mar;16(3):575-88 PMID: 110452
  49. Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein.
    Cell. 1978 Aug;14(4):921-9 PMID: 99246
  50. Sequence organization and transcription at two heat shock loci in Drosophila.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613-7 PMID: 103099
  51. Repeated gene families in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1053-63 PMID: 98263
  52. Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6694-8 PMID: 293756
  53. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  54. The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus.
    Genetics. 1979 May;92(1):279-95 PMID: 499767
  55. Translocation specificity of the Tn3 element: characterization of sites of multiple insertions.
    Cell. 1980 Jan;19(1):151-60 PMID: 6244100
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1983-09-00
Pages
5350-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384253
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]