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PMID: 16621833 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis.

Journal of bacteriology ·Vol. 188 ·No. 9 ·2006-05-00 ·Pages 3382-90

Han CS, Xie G, Challacombe JF, Altherr MR, Bhotika SS, Brown N, Bruce D, Campbell CS, Campbell ML, Chen J, Chertkov O, Cleland C, Dimitrijevic M, Doggett NA, Fawcett JJ, Glavina T, Goodwin LA, Green LD, Hill KK, Hitchcock P, Jackson PJ, Keim P, Kewalramani AR, Longmire J, Lucas S, Malfatti S, McMurry K, Meincke LJ, Misra M, Moseman BL, Mundt M, Munk AC, Okinaka RT, Parson-Quintana B, Reilly LP, Richardson P, Robinson DL, Rubin E, Saunders E, Tapia R, Tesmer JG, Thayer N, Thompson LS, Tice H, Ticknor LO, Wills PL, Brettin TS, Gilna P

Abstract

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

MeSH Terms
Amino Acids/metabolism Animals Bacillus anthracis/genetics Bacillus cereus/genetics,pathogenicity,physiology Bacillus thuringiensis/genetics Bacterial Capsules/biosynthesis,genetics Carbohydrate Metabolism Evolution, Molecular Genome, Bacterial Humans Sequence Analysis Spores, Bacterial/growth & development Virulence/genetics
Chemicals
Amino Acids
Authors & Affiliations
48 authors, click to expand affiliations / ORCID
Han Cliff S
Department of Energy Joint Genome Institute, Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Xie Gary
Challacombe Jean F
Altherr Michael R
Bhotika Smriti S
Brown Nancy
Bruce David
Campbell Connie S
Campbell Mary L
Chen Jin
Chertkov Olga
Cleland Cathy
Dimitrijevic Mira
Doggett Norman A
Fawcett John J
Glavina Tijana
Goodwin Lynne A
Green Lance D
Hill Karen K
Hitchcock Penny
Jackson Paul J
Keim Paul
Kewalramani Avinash Ramesh
Longmire Jon
Lucas Susan
Malfatti Stephanie
McMurry Kim
Meincke Linda J
Misra Monica
Moseman Bernice L
Mundt Mark
Munk A Christine
Okinaka Richard T
Parson-Quintana B
Reilly Lee Philip
Richardson Paul
Robinson Donna L
Rubin Eddy
Saunders Elizabeth
Tapia Roxanne
Tesmer Judith G
Thayer Nina
Thompson Linda S
Tice Hope
Ticknor Lawrence O
Wills Patti L
Brettin Thomas S
Gilna Paul
References (41)
41 references, click to expand
  1. PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis.
    Mol Microbiol. 1999 Jun;32(5):1043-53 PMID: 10361306
  2. Role of the gerI operon of Bacillus cereus 569 in the response of spores to germinants.
    J Bacteriol. 1998 Dec;180(24):6729-35 PMID: 9852021
  3. Identification and characterization of the gerH operon of Bacillus anthracis endospores: a differential role for purine nucleosides in germination.
    J Bacteriol. 2003 Feb;185(4):1462-4 PMID: 12562819
  4. Enterotoxic activity of hemolysin BL from Bacillus cereus.
    Infect Immun. 1995 Nov;63(11):4423-8 PMID: 7591080
  5. Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus and Bacillus thuringiensis Soil Isolates.
    Appl Environ Microbiol. 2001 Oct;67(10):4863-73 PMID: 11571195
  6. Tandem repeats finder: a program to analyze DNA sequences.
    Nucleic Acids Res. 1999 Jan 15;27(2):573-80 PMID: 9862982
  7. Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects.
    Proc Natl Acad Sci U S A. 1996 May 28;93(11):5389-94 PMID: 8643585
  8. Bacillus thuringiensis and its pesticidal crystal proteins.
    Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806 PMID: 9729609
  9. Population structure and evolution of the Bacillus cereus group.
    J Bacteriol. 2004 Dec;186(23):7959-70 PMID: 15547268
  10. Cloning and expression of the vegetative insecticidal protein (vip3V) gene of Bacillus thuringiensis in Escherichia coli.
    Protein Expr Purif. 2002 Oct;26(1):82-8 PMID: 12356474
  11. Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis.
    Nature. 2003 May 1;423(6935):87-91 PMID: 12721630
  12. The hidden lifestyles of Bacillus cereus and relatives.
    Environ Microbiol. 2003 Aug;5(8):631-40 PMID: 12871230
  13. Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons.
    Microbiology (Reading). 2002 Jul;148(Pt 7):2089-2095 PMID: 12101297
  14. The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1.
    Nucleic Acids Res. 2004 Feb 11;32(3):977-88 PMID: 14960714
  15. Intra- and interspecies signaling between Streptococcus salivarius and Streptococcus pyogenes mediated by SalA and SalA1 lantibiotic peptides.
    J Bacteriol. 2001 Jul;183(13):3931-8 PMID: 11395456
  16. Microbial gene identification using interpolated Markov models.
    Nucleic Acids Res. 1998 Jan 15;26(2):544-8 PMID: 9421513
  17. Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: case report and experimental evidence of pathogenicity in immunosuppressed mice.
    J Clin Microbiol. 1998 Jul;36(7):2138-9 PMID: 9650985
  18. Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates.
    Appl Environ Microbiol. 2004 Feb;70(2):1068-80 PMID: 14766590
  19. Improved microbial gene identification with GLIMMER.
    Nucleic Acids Res. 1999 Dec 1;27(23):4636-41 PMID: 10556321
  20. The capsule of bacillus anthracis, a review
    J Appl Microbiol. 1999 Aug;87(2):250 PMID: 10475959
  21. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis--one species on the basis of genetic evidence.
    Appl Environ Microbiol. 2000 Jun;66(6):2627-30 PMID: 10831447
  22. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.
    Nucleic Acids Res. 1997 Mar 1;25(5):955-64 PMID: 9023104
  23. Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax.
    Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8449-54 PMID: 15155910
  24. Bacillus thuringiensis serotype H34 isolated from human and insecticidal strains serotypes 3a3b and H14 can lead to death of immunocompetent mice after pulmonary infection.
    FEMS Immunol Med Microbiol. 1999 May;24(1):43-7 PMID: 10340711
  25. Correlation between specific plasmids and delta-endotoxin production in Bacillus thuringiensis.
    Plasmid. 1981 May;5(3):352-65 PMID: 7267811
  26. Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis.
    Appl Environ Microbiol. 2003 May;69(5):2755-64 PMID: 12732546
  27. Expression of vip1/vip2 genes in Escherichia coli and Bacillus thuringiensis and the analysis of their signal peptides.
    J Appl Microbiol. 2004;97(4):757-65 PMID: 15357725
  28. Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species.
    J Bacteriol. 2002 Jan;184(1):134-41 PMID: 11741853
  29. A novel Bacillus subtilis gene involved in negative control of sporulation and degradative-enzyme production.
    J Bacteriol. 1990 Apr;172(4):1783-90 PMID: 2108124
  30. Genetic aspects of bacterial endospore formation.
    Bacteriol Rev. 1976 Dec;40(4):908-62 PMID: 12736
  31. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins.
    Microbiol Mol Biol Rev. 1998 Sep;62(3):807-13 PMID: 9729610
  32. Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes.
    J Bacteriol. 1999 Oct;181(20):6509-15 PMID: 10515943
  33. Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing.
    Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13536-41 PMID: 15347815
  34. The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria.
    Nature. 2003 May 1;423(6935):81-6 PMID: 12721629
  35. Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.
    FEMS Microbiol Lett. 1989 Jul 15;51(1):211-7 PMID: 2550317
  36. Bacillus cereus and related species.
    Clin Microbiol Rev. 1993 Oct;6(4):324-38 PMID: 8269390
  37. Fast algorithms for large-scale genome alignment and comparison.
    Nucleic Acids Res. 2002 Jun 1;30(11):2478-83 PMID: 12034836
  38. PipMaker--a web server for aligning two genomic DNA sequences.
    Genome Res. 2000 Apr;10(4):577-86 PMID: 10779500
  39. Phenotypic and genotypic comparisons of 23 strains from the Bacillus cereus complex for a selection of known and putative B. thuringiensis virulence factors.
    FEMS Microbiol Lett. 2000 Jul 1;188(1):7-13 PMID: 10867226
  40. The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects.
    Microbiology (Reading). 2000 Nov;146 ( Pt 11):2825-2832 PMID: 11065361
  41. Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis.
    J Bacteriol. 2003 Feb;185(4):1443-54 PMID: 12562816
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.188.9.3382-3390.2006
Published
2006-05-00
Pages
3382-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1447445
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/JB.188.9.3382-3390.2006 PMC Full Text View journal details
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