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PMID: 1662362 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

P sequences of Drosophila subobscura lack exon 3 and may encode a 66 kd repressor-like protein.

Nucleic acids research ·Vol. 19 ·No. 24 ·1991-12-25 ·Pages 6713-8

Paricio N, Pérez-Alonso M, Martínez-Sebastián MJ, de Frutos R

Abstract

Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These sequences are located at the 85DE region of the O chromosome and at least three of them are organized in tandem. We have identified four copies which exhibit strong similarity between them. All of the isolated elements are truncated at the 5' and 3' ends. They have lost the inverted terminal repeats and exon 3, but maintain exons 0, 1 and 2. They are transcribed producing a polyadenylated RNA. The structure of these transcripts suggests that they are able to encode a 66 kd repressor-like protein, but not a functional transposase. We ask about the biological role of a potential repressor protein in this species.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Transposable Elements/genetics Drosophila/genetics Exons/genetics Leucine Zippers/genetics Molecular Sequence Data Nucleic Acid Hybridization Nucleotidyltransferases/genetics Repetitive Sequences, Nucleic Acid/genetics Repressor Proteins/genetics Restriction Mapping Sequence Homology, Nucleic Acid Transposases
Chemicals
DNA Transposable Elements Repressor Proteins Nucleotidyltransferases Transposases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paricio N
Department of Genetics, University of Valencia, Spain.
Pérez-Alonso M
Martínez-Sebastián M J
de Frutos R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.24.6713
Published
1991-12-25
Pages
6713-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329299
Subset
IM
Databases
GENBANK
S74793, S74795, X60436, X60437, X60655, X60963, X60964, X60965, X60966, X60967, X60968, X60969
External Links
PubMed source DOI: 10.1093/nar/19.24.6713 PMC Full Text View journal details
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