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PMID: 16668040 Published · ppublish English Journal Article

Expression of DNA coding for diphtheria toxin chain a is toxic to plant cells.

Plant physiology ·Vol. 95 ·No. 3 ·1991-03-00 ·Pages 687-92

Czako M, An G

Abstract

DNA coding for the enzymatically active subunit A of diphtheria toxin was placed under the control of the cauliflower mosaic virus 35S promoter and the Agrobacterium left transfer-DNA gene 7 polyadenylation signal. Agrobacteria carrying a binary plant vector with the chimeric diphtheria toxin A gene had very low transforming activity in tobacco (Nicotiana tabacum L.), and greatly diminished the recovery of stable transformants when mixed together with agrobacteria which alone transformed plant cells well. The introduction of this chimeric molecule into tobacco cells by electroporation lowered the level of the transient expression of the coelectroporated chloramphenicol acetyltransferase reporter gene indicating that expression of diphtheria toxin chain A in plant cells is toxic. We have developed a binary vector pGA987 which can be used for probing a variety of plant promoters.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Czako M
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
An G
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.95.3.687
Published
1991-03-00
Pages
687-92
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1077592
External Links
PubMed source DOI: 10.1104/pp.95.3.687 PMC Full Text View journal details
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