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PMID: 16742825 Published · ppublish English Journal Article

Determination and significance of l-tyrosine O-sulphate and its deaminated metabolites in normal human and mouse urine.

The Biochemical journal ·Vol. 134 ·No. 2 ·1973-06-00 ·Pages 629-35

Hext PM, Thomas S, Rose FA, Dodgson KS

Abstract

Methods were developed for the determination of the O-sulphate esters of l-tyrosine, p-hydroxyphenylpyruvate, p-hydroxyphenylacetate and p-hydroxybenzaldehyde in human urine. The amounts of these esters normally present in human female urine were determined. The quantities and specific radioactivities of l-tyrosine O-sulphate and p-hydroxyphenylpyruvate O-sulphate in mouse urine after labelled tyrosine had been fed were determined and were consistent with the hypothesis that the sole source of p-hydroxyphenylpyruvate O-sulphate was l-tyrosine O-sulphate. It is suggested that the turnover of known polypeptides containing l-tyrosine O-sulphate residues can account for only a portion of the quantities of the ester and its metabolite(s) that are present in normal female human urine.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hext P M
Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, U.K.
Thomas S
Rose F A
Dodgson K S
References (25)
25 references, click to expand
  1. A rat-tissue aminotransferase acting on L-tyrosine O-sulphate.
    Biochem J. 1966 Jan;98(1):168-72 PMID: 4287183
  2. Determination of metabolites of tyrosine and of tryptophan and related compounds by gas-liquid chromatography.
    J Chromatogr. 1971 Mar 3;55(2):297-302 PMID: 5102184
  3. Aromatic acid excretion in phenylketonuria. Analysis of the unconjugated aromatic acids derived from phenylalanine.
    Clin Chim Acta. 1970 Jan;27(1):5-18 PMID: 5412612
  4. Partial purification and properties of an enzyme from rat liver that catalyses the sulphation of L-tyrosyl derivatives.
    Biochem J. 1970 Mar;116(5):797-803 PMID: 5441369
  5. The effect of substrate concentration and pH on the enzymic sulphation of L-tyrosyl derivatives.
    Biochem J. 1970 Mar;116(5):805-10 PMID: 5441370
  6. Alteration in tyramine metabolism by ethanol.
    Biochem Pharmacol. 1970 Feb;19(2):604-7 PMID: 5507668
  7. Structure of porcine cholecystokinin-pancreozymin. 1. Cleavage with thrombin and with trypsin.
    Eur J Biochem. 1968 Oct 17;6(1):156-62 PMID: 5725809
  8. The biological sulphation of L-tyrosyl peptides.
    Biochem J. 1966 Jan;98(1):138-41 PMID: 5938633
  9. A possible route to the production of free L-tyrosine O-sulphate by the rat.
    Biochem J. 1966 Jun;99(3):534-7 PMID: 5965096
  10. The detection and determination of L-tyrosine O-sulphate in rabbit and other mammalian urine.
    Biochem J. 1966 Jul;100(1):278-81 PMID: 5965255
  11. Gas-liquid chromatographic study and estimation of several urinary aromatic acids.
    Anal Biochem. 1966 Nov;17(2):244-57 PMID: 5971420
  12. Studies on sulphatases. VIII. The arylsulphatase of a strain of Alcaligenes metalcaligenes isolated from intertidal mud.
    Biochem J. 1954 Oct;58(2):182-7 PMID: 13208569
  13. Tyrosine-O-sulfate as a constituent of normal human urine.
    J Biol Chem. 1955 Dec;217(2):703-8 PMID: 13271432
  14. Separation of steroid sulfokinases.
    J Biol Chem. 1958 Dec;233(6):1348-51 PMID: 13610840
  15. Studies on sulphatases. 23. The enzymic desulphation of tyrosine O-sulphate.
    Biochem J. 1959 Jan;71(1):10-5 PMID: 13628524
  16. Enzymatic sulfurylation of tyrosine derivatives.
    J Biol Chem. 1959 Apr;234(4):909-11 PMID: 13654288
  17. Observations on the metabolism of tyrosine O[35S]-sulphate in the rat.
    Biochem J. 1961 May;79:209-13 PMID: 13723348
  18. Synthesis of 35S-labelled arylsulphates byintact animals and by tissue preparations, with particular reference to L-tyrosine O-sulphate.
    Biochem J. 1959 Dec;73:723-9 PMID: 13829369
  19. Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides.
    J Biol Chem. 1960 Feb;235:257-66 PMID: 13835879
  20. Studies on L-tyrosine O-sulphate. 3. The metabolic fate of the L-tyrosine O[35S]-sulphate residue of 35S-labelled rabbit fibrinopeptide B.
    Biochem J. 1963 Jun;87:548-53 PMID: 14041998
  21. TYROSINE O-SULPHATE IN FIBRINOGEN AND FIBRIN.
    Biochem J. 1963 Dec;89:621-4 PMID: 14101983
  22. THE ANTRAL HORMONE GASTRIN. STRUCTURE OF GASTRIN.
    Nature. 1964 Dec 5;204:931-3 PMID: 14248711
  23. BIOSYNTHESIS OF L-TYROSINE O-SULPHATE FROM THE METHYL AND ETHYL ESTERS OF L-TYROSINE.
    Biochem J. 1965 Feb;94:331-6 PMID: 14348193
  24. Studies on l-tyrosine O-sulphate. 1. Preparation and properties of potassium p-hydroxyphenylpyruvic acid [S]sulphate.
    Biochem J. 1963 Jun;87(3):541-5 PMID: 16749015
  25. Studies on l-tyrosine O-sulphate. 2. Identification of p-hydroxyphenylpyruvic acid [S]sulphate as a metabolite of l-tyrosine O[S]-sulphate in the rat.
    Biochem J. 1963 Jun;87(3):545-8 PMID: 16749016
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1340629
Published
1973-06-00
Pages
629-35
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177851
External Links
PubMed source DOI: 10.1042/bj1340629 PMC Full Text View journal details
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