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PMID: 16782869 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 26 ·No. 13 ·2006-07-00 ·Pages 4794-805

Chou S, Lane S, Liu H

Abstract

The Saccharomyces cerevisiae transcription factor Ste12 controls two distinct developmental programs of mating and filamentation. Ste12 activity is regulated by Fus3 and Kss1 mitogen-activated protein kinases through two Ste12 inhibitors, Dig1 and Dig2. Mating genes are regulated by Ste12 through Ste12 binding sites (pheromone response elements [PREs]), whereas filamentation genes are supposedly regulated by the cooperative binding of Ste12 and Tec1 on a PRE adjacent to a Tec1-binding site (TCS), termed filamentous responsive element (FRE). However, most filamentation genes do not contain an FRE; instead, they all have a TCS. By immunoprecipitation, we show that Ste12 forms two distinct complexes, Ste12/Dig1/Dig2 and Tec1/Ste12/Dig1, both in vivo and in vitro. The two complexes are formed by the competitive binding of Tec1 and Dig2 with Ste12, as Tec1 can compete off Dig2 from Ste12 in vitro and in vivo. In the Tec1/Ste12/Dig1 complex, Tec1 binds to the N terminus of Ste12 and to Dig1 indirectly through Ste12. Tec1 has low basal activity, and its transcriptional activation is provided by the associated Ste12, which is under Dig1 inhibition. Filamentation genes are bound by the Tec1/Ste12/Dig1 complex, whereas mating genes are occupied by mostly Ste12/Dig1/Dig2 with some Tec1/Ste12/Dig1. We suggest that Tec1 tethers Ste12 to TCS elements upstream of filamentation genes and defines the filamentation genes as a subset of Ste12-regulated genes.

MeSH Terms
Binding, Competitive Chromatin Immunoprecipitation DNA-Binding Proteins/antagonists & inhibitors,metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Genes, Mating Type, Fungal Promoter Regions, Genetic Response Elements Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/antagonists & inhibitors,genetics,metabolism Transcription Factors/antagonists & inhibitors,genetics,metabolism Transcription, Genetic
Chemicals
DIG1 protein, S cerevisiae DIG2 protein, S cerevisiae DNA-Binding Proteins STE12 protein, S cerevisiae Saccharomyces cerevisiae Proteins TEC1 protein, S cerevisiae Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chou Song
Department of Biological Chemistry, University of California-Irvine, Irvine, CA 92697-1700, USA.
Lane Shelley
Liu Haoping
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46 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.02053-05
Published
2006-07-00
Pages
4794-805
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1489142
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055155 · United States
NIGMS NIH HHS · GM55155 · United States
External Links
PubMed source DOI: 10.1128/MCB.02053-05 PMC Full Text View journal details
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