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PMID: 16789222 Published · ppublish English Journal Article

Genome of Bacillus subtilis Bacteriophage SPP1: Structure and Nucleotide Sequence of pac, the Origin of DNA Packaging.

Journal of virology ·Vol. 42 ·No. 1 ·1982-04-00 ·Pages 83-90

Deichelbohrer I, Messer W, Trautner TA

Abstract

The DNA of Bacillus subtilis bacteriophage SPP1 is terminally redundant and partially circularly permuted. To explain these parameters, we followed the Streisinger-Botstein models of phage maturation and assumed that packaging of SPP1 DNA begins at a unique genomic site ("pac") and proceeds sequentially from there. We describe the sequence of about 1,000 nucleotides surrounding pac. This together with size determinations of small, pac-terminated restriction fragments has revealed heterogeneity of the natural pac ends of SPP1 DNA. Such ends fell in each DNA strand into a region of five to seven nucleotides. However, within this range more than 50% of all molecules terminated with defined cytosines on both strands, generating a 3' protruding terminus. The nucleotide sequence of the DNA segment surrounding pac did not reveal any features which would distinguish this region.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deichelbohrer I
Max-Planck-Institut für Molekulare Genetik, Abteilung Trautner, D-1000 Berlin 33, Germany.
Messer W
Trautner T A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.1.83-90.1982
Published
1982-04-00
Pages
83-90
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256047
External Links
PubMed source DOI: 10.1128/JVI.42.1.83-90.1982 PMC Full Text View journal details
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