Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.

Journal of bacteriology ·Vol. 173 ·No. 23 ·1991-12-00 ·Pages 7728-31

Frost LS, Bazett-Jones DP

Abstract

F-like pili specified by conjugative plasmids have been reported to contain phosphate which may be noncovalently incorporated into the pilus. Electron spectroscopic imaging was able to detect phosphate in the filamentous, single-stranded DNA phage f1, used as positive control, but could not detect phosphate in F-like pili. Thus, the phosphate in purified pili which has been reported is probably derived from contaminating cell envelope material.

MeSH Terms

Coliphages/ultrastructure Conjugation, Genetic Escherichia coli/genetics,physiology,ultrastructure Fimbriae, Bacterial/physiology,ultrastructure Microscopy, Electron/methods Phosphates/analysis Plasmids

Chemicals

Phosphates

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Frost L S

Department of Microbiology, University of Alberta, Edmonton, Canada.

Bazett-Jones D P

References (27)

27 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.173.23.7728-7731.1991

Published

1991-12-00

Pages

7728-31

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212548

Subset

External Links

PubMed source DOI: 10.1128/jb.173.23.7728-7731.1991 PMC Full Text View journal details

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Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.

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