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PMID: 16880522 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Isw1 functions in parallel with the NuA4 and Swr1 complexes in stress-induced gene repression.

Molecular and cellular biology ·Vol. 26 ·No. 16 ·2006-08-00 ·Pages 6117-29

Lindstrom KC, Vary JC, Parthun MR, Delrow J, Tsukiyama T

Abstract

The packaging of DNA into chromatin allows eukaryotic cells to organize and compact their genomes but also creates an environment that is generally repressive to nuclear processes that depend upon DNA accessibility. There are several classes of enzymes that modulate the primary structure of chromatin to regulate various DNA-dependent processes. The biochemical activities of the yeast Isw1 ATP-dependent chromatin-remodeling enzyme have been well characterized in vitro, but little is known about how these activities are utilized in vivo. In this work, we sought to discern genetic backgrounds that require Isw1 activity for normal growth. We identified a three-way genetic interaction among Isw1, the NuA4 histone acetyltransferase complex, and the Swr1 histone replacement complex. Transcription microarray analysis revealed parallel functions for these three chromatin-modifying factors in the regulation of TATA-containing genes, including the repression of a large number of stress-induced genes under normal growth conditions. In contrast to a recruitment-based model, we find that the NuA4 and Swr1 complexes act throughout the genome while only a specific subset of the genome shows alterations in transcription.

MeSH Terms
Acetylation Acetyltransferases/deficiency,metabolism Adenosine Triphosphatases/deficiency,metabolism Chromatin Assembly and Disassembly/genetics DNA-Binding Proteins/deficiency,metabolism Down-Regulation/drug effects Gene Expression Regulation, Fungal/drug effects Genome, Fungal/genetics Histone Acetyltransferases Histones/metabolism Lysine/metabolism Microbial Sensitivity Tests Mutation/genetics Promoter Regions, Genetic/genetics Protein Transport Saccharomyces cerevisiae/cytology,drug effects,enzymology Saccharomyces cerevisiae Proteins/metabolism Sirolimus/pharmacology Transcription Factors/metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Histones MSN2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Acetyltransferases Histone Acetyltransferases NuA4 protein, S cerevisiae Adenosine Triphosphatases ISW1 protein, S cerevisiae Swr1 protein, S cerevisiae Lysine Sirolimus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lindstrom Kimberly C
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Vary Jay C
Parthun Mark R
Delrow Jeffrey
Tsukiyama Toshio
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00642-06
Published
2006-08-00
Pages
6117-29
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1592817
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058465 · United States
NIGMS NIH HHS · R01 GM062970 · United States
NIGMS NIH HHS · GM58465 · United States
NIGMS NIH HHS · GM62970 · United States
External Links
PubMed source DOI: 10.1128/MCB.00642-06 PMC Full Text View journal details
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