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PMID: 14701747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Eaf3 regulates the global pattern of histone acetylation in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 24 ·No. 2 ·2004-01-00 ·Pages 757-64

Reid JL, Moqtaderi Z, Struhl K

Abstract

Saccharomyces cerevisiae has a global pattern of histone acetylation in which histone H3 and H4 acetylation levels are lower at protein-coding sequences than at promoter regions. The loss of Eaf3, a subunit of the NuA4 histone acetylase and Rpd3 histone deacetylase complexes, greatly alters the genomic profile of histone acetylation, with the effects on H4 appearing to be more pronounced than those on H3. Specifically, the loss of Eaf3 causes increases in H3 and H4 acetylation at coding sequences and decreases at promoters, such that histone acetylation levels become evenly distributed across the genome. Eaf3 does not affect the overall level of H4 acetylation, the recruitment of the NuA4 catalytic subunit Esa1 to target promoters, or the level of transcription of the genes analyzed for histone acetylation. Whole-genome transcriptional profiling indicates that Eaf3 plays a positive, but quantitatively modest, role in the transcription of a small subset of genes, whereas it has a negative effect on very few genes. We suggest that Eaf3 regulates the genomic profile of histone H3 and H4 acetylation in a manner that does not involve targeted recruitment and is independent of transcriptional activity.

MeSH Terms
Acetylation Acetyltransferases/genetics,metabolism Gene Deletion Genome, Fungal Histone Acetyltransferases Histones/chemistry,genetics,metabolism Promoter Regions, Genetic Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription, Genetic
Chemicals
Histones Ribosomal Proteins Saccharomyces cerevisiae Proteins Acetyltransferases Eaf3 protein, S cerevisiae Esa1 protein, S cerevisiae Histone Acetyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reid Juliet L
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Moqtaderi Zarmik
Struhl Kevin
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.2.757-764.2004
Published
2004-01-00
Pages
757-64
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC343795
Subset
IM
Grants
NIGMS NIH HHS · GM 30186 · United States
NIGMS NIH HHS · R01 GM030186 · United States
NIGMS NIH HHS · R37 GM030186 · United States
NIGMS NIH HHS · R01 GM053720 · United States
NIGMS NIH HHS · GM 53720 · United States
External Links
PubMed source DOI: 10.1128/MCB.24.2.757-764.2004 PMC Full Text View journal details
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