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PMID: 16880537 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bloom helicase and DNA topoisomerase IIIalpha are involved in the dissolution of sister chromatids.

Molecular and cellular biology ·Vol. 26 ·No. 16 ·2006-08-00 ·Pages 6299-307

Seki M, Nakagawa T, Seki T, Kato G, Tada S, Takahashi Y, Yoshimura A, Kobayashi T, Aoki A, Otsuki M, Habermann FA, Tanabe H, Ishii Y, Enomoto T

Abstract

Bloom's syndrome (BS) is an autosomal disorder characterized by predisposition to a wide variety of cancers. The gene product whose mutation leads to BS is the RecQ family helicase BLM, which forms a complex with DNA topoisomerase IIIalpha (Top3alpha). However, the physiological relevance of the interaction between BLM and Top3alpha within the cell remains unclear. We show here that Top3alpha depletion causes accumulation of cells in G2 phase, enlargement of nuclei, and chromosome gaps and breaks that occur at the same position in sister chromatids. The transition from metaphase to anaphase is also inhibited. All of these phenomena except cell lethality are suppressed by BLM gene disruption. Taken together with the biochemical properties of BLM and Top3alpha, these data indicate that BLM and Top3alpha execute the dissolution of sister chromatids.

MeSH Terms
2-Aminopurine/pharmacology Adenosine Triphosphatases/metabolism Anaphase/drug effects Animals Apoptosis Chickens Chromatids/drug effects,enzymology,genetics Chromosome Aberrations DNA Helicases/metabolism DNA Topoisomerases, Type I/deficiency,metabolism G2 Phase/drug effects Gene Targeting Humans Isoenzymes/metabolism Metaphase/drug effects Mice Models, Genetic Mutation/genetics Phenotype RecQ Helicases
Chemicals
Isoenzymes 2-Aminopurine Adenosine Triphosphatases Bloom syndrome protein DNA Helicases RecQ Helicases DNA Topoisomerases, Type I
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Seki Masayuki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan. [email protected]
Nakagawa Takayuki
Seki Takahiko
Kato Genta
Tada Shusuke
Takahashi Yuriko
Yoshimura Akari
Kobayashi Takayuki
Aoki Ayako
Otsuki Makoto
Habermann Felix A
Tanabe Hideyuki
Ishii Yutaka
Enomoto Takemi
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00702-06
Email
[email protected]
Published
2006-08-00
Pages
6299-307
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1592785
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.00702-06 PMC Full Text View journal details
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