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PMID: 1688465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The product of the H19 gene may function as an RNA.

Molecular and cellular biology ·Vol. 10 ·No. 1 ·1990-01-00 ·Pages 28-36

Brannan CI, Dees EC, Ingram RS, Tilghman SM

Abstract

The mouse H19 gene was identified as an abundant hepatic fetal-specific mRNA under the transcriptional control of a trans-acting locus termed raf. The protein this gene encoded was not apparent from an analysis of its nucleotide sequence, since the mRNA contained multiple translation termination signals in all three reading frames. As a means of assessing which of the 35 small open reading frames might be important to the function of the gene, the human H19 gene was cloned and sequenced. Comparison of the two homologs revealed no conserved open reading frame. Cellular fractionation showed that H19 RNA is cytoplasmic but not associated with the translational machinery. Instead, it is located in a particle with a sedimentation coefficient of approximately 28S. Despite the fact that it is transcribed by RNA polymerase II and is spliced and polyadenylated, we suggest that the H19 RNA is not a classical mRNA. Instead, the product of this unusual gene may be an RNA molecule.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cytoplasm DNA/genetics Genes Humans Mice Molecular Sequence Data Protein Biosynthesis Proteins/genetics RNA/genetics,metabolism RNA Polymerase II/metabolism Ribosomes/metabolism Transcription, Genetic
Chemicals
Proteins RNA DNA RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brannan C I
Howard Hughes Medical Institute, Princeton University New Jersey 08544.
Dees E C
Ingram R S
Tilghman S M
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.1.28-36.1990
Published
1990-01-00
Pages
28-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360709
Subset
IM
Grants
NCI NIH HHS · CA44976 · United States
Databases
GENBANK
M32053
External Links
PubMed source DOI: 10.1128/mcb.10.1.28-36.1990 PMC Full Text View journal details
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