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PMID: 1689056 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and structure of an arrestin gene from Drosophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 3 ·1990-02-00 ·Pages 1003-7

Smith DP, Shieh BH, Zuker CS

Abstract

A Drosophila gene encoding a homologue of vertebrate arrestin was isolated by subtractive hybridization and identified as a member of a set of genes that are preferentially expressed in the visual system. This gene encodes a 364-amino acid protein that displays greater than 40% amino acid sequence identity with human and bovine arrestin. Interestingly, the Drosophila homologue lacks the C-terminal sequences that were postulated to interact with rhodopsin during the quenching of the phototransduction cascade in the vertebrate visual response. These findings are discussed in terms of invertebrate phototransduction. The Drosophila gene was mapped cytogenetically to chromosomal position 36D1-2, near the ninaD locus. However, the arrestin gene does not appear to be the ninaD locus, as sequence analysis of three ethylmethane sulfate-induced ninaD mutant alleles reveals no alteration in amino acid sequence.

MeSH Terms
Alleles Amino Acid Sequence Animals Antigens/genetics Arrestin Base Sequence DNA/genetics,isolation & purification Drosophila/genetics Eye Proteins/genetics Genes Head Membrane Proteins/genetics Molecular Sequence Data Nucleic Acid Hybridization Phosphodiesterase Inhibitors/metabolism RNA/genetics,isolation & purification Restriction Mapping Salivary Glands/cytology Sequence Homology, Nucleic Acid
Chemicals
Antigens Arrestin Eye Proteins Membrane Proteins Phosphodiesterase Inhibitors RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith D P
Howard Hughes Medical Institute, University of California at San Diego, La Jolla 92093.
Shieh B H
Zuker C S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.3.1003
Published
1990-02-00
Pages
1003-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53398
Subset
IM
Databases
GENBANK
M30177
External Links
PubMed source DOI: 10.1073/pnas.87.3.1003 PMC Full Text View journal details
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