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PMID: 3478675 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Primary and secondary structure of bovine retinal S antigen (48-kDa protein).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 20 ·1987-10-00 ·Pages 6975-9

Shinohara T, Dietzschold B, Craft CM, Wistow G, Early JJ, Donoso LA, Horwitz J, Tao R

Abstract

The complete amino acid sequence of bovine S antigen (48-kDa protein) has been determined by cDNA and partial amino acid sequencing. A 1623-base-pair (bp) cDNA contains an open reading frame coding for a protein of 404 amino acids (45,275 Da). Tryptic peptides and cyanogen bromide peptides of native bovine S antigen were purified and partially sequenced. All of these peptides were accounted for in the long open reading frame. Searching of the National Biomedical Research Foundation data bank revealed no extensive sequence homology between S antigen and other proteins. However, there are local regions of sequence similarity with alpha transducin, including the sites subject to ADP-ribosylation by Bordetella pertussis and cholera toxins and the phosphoryl binding-sites. Secondary structure prediction and circular dichroic spectroscopy show that S antigen is composed predominantly of beta-sheet conformation. Acid-catalyzed methanolysis suggests the presence of low levels of carbohydrate in the molecule.

MeSH Terms
Amino Acid Sequence Antigens Arrestin Base Sequence Circular Dichroism DNA/genetics Eye Proteins Glycoproteins Molecular Sequence Data Protein Conformation Retina/immunology Sequence Homology, Nucleic Acid
Chemicals
Antigens Arrestin Eye Proteins Glycoproteins DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Shinohara T
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20892.
Dietzschold B
Craft C M
Wistow G
Early J J
Donoso L A
Horwitz J
Tao R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.20.6975
Published
1987-10-00
Pages
6975-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299211
Subset
IM
Grants
NEI NIH HHS · EY5095 · United States
Databases
GENBANK
J02955
External Links
PubMed source DOI: 10.1073/pnas.84.20.6975 PMC Full Text View journal details
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