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PMID: 1692081 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.

The Journal of experimental medicine ·Vol. 171 ·No. 5 ·1990-05-01 ·Pages 1649-64

Mandrell RE, Lesse AJ, Sugai JV, Shero M, Griffiss JM, Cole JA, Parsons NJ, Smith H, Morse SA, Apicella MA

Abstract

After growth of gonococci in the presence of cytidine monophospho-N-acetyl-neuraminic acid (CMP-NANA), their 4.5-kD lipooligosaccharide (LOS) component was increased by approximately 400 daltons, whereas the LOS of strains lacking the 4.5-kD component were unaffected. Expression of mAb-defined epitopes on the 4.5-kD component was decreased on LOS of strains grown in CMP-NANA, and treatment of the LOS with neuraminidase reversed this affect. Gonococci incubated with human PMNs also had decreased expression of the 4.5-kD+ epitopes. A detergent extract of gonococci incorporated radiolabeled NANA in the LOS, suggesting the presence of a sialyltransferase in gonococci. Exogenous sialyltransferases also could use LOS as an acceptor.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Bacterial/immunology Cytidine Monophosphate N-Acetylneuraminic Acid/metabolism Electrophoresis, Polyacrylamide Gel Epitopes/immunology Humans Immunoblotting Lipopolysaccharides/immunology,isolation & purification Microscopy, Electron Neisseria gonorrhoeae/immunology,ultrastructure Neuraminidase Neutrophils/microbiology Radioimmunoassay Sialic Acids/metabolism Submandibular Gland/enzymology Swine
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Epitopes Lipopolysaccharides Sialic Acids lipid-linked oligosaccharides Cytidine Monophosphate N-Acetylneuraminic Acid Neuraminidase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Mandrell R E
Center for Immunochemistry, Veterans Administration Medical Center, San Francisco, California 94121.
Lesse A J
Sugai J V
Shero M
Griffiss J M
Cole J A
Parsons N J
Smith H
Morse S A
Apicella M A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.171.5.1649
Published
1990-05-01
Pages
1649-64
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187906
Subset
IM
Grants
NIAID NIH HHS · AI-18384 · United States
NIAID NIH HHS · AI-21620 · United States
NIAID NIH HHS · P01 AI-21912 · United States
External Links
PubMed source DOI: 10.1084/jem.171.5.1649 PMC Full Text View journal details
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