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PMID: 16927203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance.

Plant molecular biology ·Vol. 61 ·No. 6 ·2006-08-00 ·Pages 897-915

Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK

Abstract

A novel pathogen-induced gene encoding the RAV (Related to ABI3/VP1) transcription factor, CARAV1, was isolated from pepper leaves infected with Xanthomonas campestris pv. vesicatoria. CARAV1 contains two distinct DNA-binding domains AP2 and B3 uniquely found in higher plants. Transient expression analysis of the smGFP:CARAV1 fusion construct in Arabidopsis protoplasts and pepper epidermal cells revealed the CARAV1 protein to be localized in the nucleus. The N-terminal region of CARAV1 fused to the GAL4 DNA-binding domain was required to activate transcription of reporter genes in yeast. In yeast one-hybrid, the recognition of CAACA and CACCTG motifs also were essential for the CARAV1 protein to bind to a specific target gene and activate the reporter gene. The expression of the CARAV1 gene was strongly induced early in pepper leaves during the pathogen infection, abiotic elicitors and environmental stresses. CARAV1 transcripts were localized in the phloem cells of leaf tissues during pathogen infection and ethylene treatment. Ectopic expression of the CARAV1 gene in transgenic Arabidopsis plants induced some PR genes and enhanced resistance against infection by Pseudomonas syringae pv. tomato DC3000 and osmotic stresses by high salinity and dehydration. The CARAV1 promoter activation was induced by P. syringae pv. tabaci, salicylic acid and abscisic acid. These data suggest that pathogen- and abiotic stress-inducible CARAV1 functions as a transcriptional activator triggering resistance to bacterial infection and tolerance to osmotic stresses.

MeSH Terms
Abscisic Acid/pharmacology Acetates/pharmacology Adaptation, Physiological/genetics Amino Acid Sequence Arabidopsis/drug effects,genetics,microbiology Capsicum/drug effects,genetics,microbiology Cyclopentanes/pharmacology DNA-Binding Proteins/genetics Ethylenes/pharmacology Gene Expression Regulation, Plant/drug effects,genetics Hydrogen Peroxide/pharmacology Immunity, Innate/genetics In Situ Hybridization Mannitol/pharmacology Molecular Sequence Data Oxylipins Plant Proteins/genetics Plants, Genetically Modified Pseudomonas syringae/growth & development RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sodium Chloride/pharmacology Transcription Factors/genetics Water/metabolism,pharmacology Xanthomonas campestris/growth & development
Chemicals
Acetates Cyclopentanes DNA-Binding Proteins Ethylenes Oxylipins Plant Proteins RNA, Messenger Transcription Factors Water Mannitol Sodium Chloride Abscisic Acid methyl jasmonate ethylene Hydrogen Peroxide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sohn Kee Hoon
Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of Korea.
Lee Sung Chul
Jung Ho Won
Hong Jeum Kyu
Hwang Byung Kook
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-006-0057-0
Published
2006-08-00
Pages
897-915
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF478458
External Links
PubMed source DOI: 10.1007/s11103-006-0057-0 View journal details
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