Home LiteratureArticle Details
PMID: 1692882 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Patch-clamp profile of ion channels in resting murine B lymphocytes.

The Journal of membrane biology ·Vol. 114 ·No. 2 ·1990-03-00 ·Pages 175-88

McCann FV, McCarthy DC, Noelle RJ

Abstract

Patch-clamp studies of single ion channel currents in freshly isolated murine B lymphocytes are characterized here according to their respective unitary conductances, ion selectivities, regulatory factors, distributions and kinetic behavior. The most prevalent ion channel in murine B lymphocytes is a large conductance (348 pS) nonselective anion channel. This report characterizes additional conductances including: two chloride channels (40 and 128 pS), a calcium-activated potassium channel (93 pS), and an outwardly rectifying potassium channel which displays two distinct conductances (18 and 30 pS). Like the anion channel, both chloride channels exhibit little activity in the cell-attached patch configuration. The kinetic behavior of all of these channels is complex, with variable periods of bursting and flickering activity interspersed between prolonged closed/open intervals (dwell times). It is likely that some of these channels play an important role in the signal transduction of B cell activation.

MeSH Terms
Animals B-Lymphocytes/physiology Calcium/pharmacology Cells, Cultured Chloride Channels Chlorides/metabolism,physiology Electric Conductivity Ion Channels/physiology Kinetics Membrane Potentials Membrane Proteins/physiology Mice Mice, Inbred C57BL Mice, Inbred DBA Potassium Channels/physiology
Chemicals
Chloride Channels Chlorides Ion Channels Membrane Proteins Potassium Channels Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCann F V
Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03756.
McCarthy D C
Noelle R J
References (57)
57 references, click to expand
  1. Patch-clamp studies in human macrophages: single-channel and whole-cell characterization of two K+ conductances.
    J Membr Biol. 1988 Jul;103(1):55-66 PMID: 2460627
  2. A voltage-gated potassium channel in human T lymphocytes.
    J Physiol. 1985 Jan;358:197-237 PMID: 2580081
  3. Multi-barrelled K channels in renal tubules.
    Nature. 1987 Jun 11-17;327(6122):522-4 PMID: 2438559
  4. Phagocytosis by human macrophages is accompanied by changes in ionic channel currents.
    J Cell Biol. 1988 Jun;106(6):1873-8 PMID: 2454928
  5. A computer method for the acquisition and analysis of patch-clamp single-channel currents.
    J Neurosci Methods. 1987 May;20(1):45-55 PMID: 2438521
  6. Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis?
    Nature. 1984 Feb 2-8;307(5950):465-8 PMID: 6320007
  7. Amiloride-sensitive Na channels from the apical membrane of the rat cortical collecting tubule.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2767-70 PMID: 2422661
  8. Ion channels in human macrophages compared with the U-937 cell line.
    J Membr Biol. 1987;96(1):57-64 PMID: 2438414
  9. Ion conductance and selectivity of single calcium-activated potassium channels in cultured rat muscle.
    J Gen Physiol. 1984 Jul;84(1):1-23 PMID: 6086805
  10. Inhibition of the generation of cytotoxic lymphocytes by potassium ion channel blockers.
    Immunology. 1988 Sep;65(1):101-5 PMID: 2846432
  11. Variation of calcium current during the cell growth cycle in mouse hybridoma lines secreting immunoglobulins.
    J Physiol. 1984 Oct;355:313-21 PMID: 6491993
  12. Volume-induced anion conductance in human B lymphocytes is cation independent.
    Am J Physiol. 1983 Jul;245(1):C160-3 PMID: 6191575
  13. Mitogen induction of ion channels in murine T lymphocytes.
    J Gen Physiol. 1987 Mar;89(3):405-20 PMID: 2435845
  14. A large anion-selective channel has seven conductance levels.
    Nature. 1986 Jan 2-8;319(6048):58-60 PMID: 2417122
  15. Subset-specific expression of potassium channels in developing murine T lymphocytes.
    Science. 1988 Feb 12;239(4841 Pt 1):771-5 PMID: 2448877
  16. Single-channel recordings of apical membrane chloride conductance in A6 epithelial cells.
    J Membr Biol. 1984;80(1):81-9 PMID: 6090667
  17. Single-channel and whole-cell recordings of mitogen-regulated inward currents in human cloned helper T lymphocytes.
    Nature. 1986 Sep 18-24;323(6085):269-73 PMID: 2429188
  18. Cyclic AMP-modulated potassium channels in murine B cells and their precursors.
    Science. 1987 Mar 6;235(4793):1211-4 PMID: 2434998
  19. Characterization of a large conductance non-selective anion channel in B lymphocytes.
    Cell Signal. 1989;1(1):31-44 PMID: 2484331
  20. Volume response of quiescent and interleukin 2-stimulated T-lymphocytes to hypotonicity.
    Am J Physiol. 1988 Feb;254(2 Pt 1):C286-96 PMID: 3258129
  21. Dual effects of serotonin on a voltage-gated conductance in lymphocytes.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4557-61 PMID: 3260036
  22. Enhancement of voltage-gated Na+ channel current associated with multidrug resistance in human leukemia cells.
    Cancer Res. 1987 Jul 15;47(14):3736-41 PMID: 2439197
  23. Studies on the calcium dependence of human NK cell killing.
    Biochem Pharmacol. 1987 Nov 15;36(22):3943-9 PMID: 2446626
  24. Changes in the expression of potassium channels during mouse T cell development.
    J Exp Med. 1986 Dec 1;164(6):1846-61 PMID: 2431091
  25. Kinetics of voltage- and Ca2+ activation and Ba2+ blockade of a large-conductance K+ channel from Necturus enterocytes.
    J Membr Biol. 1988 Oct;105(1):65-75 PMID: 3225837
  26. Voltage-gated potassium conductance in human T lymphocytes stimulated with phorbol ester.
    J Physiol. 1986 Mar;372:405-23 PMID: 3487642
  27. Single calcium-dependent potassium channels in clonal anterior pituitary cells.
    Biophys J. 1982 Sep;39(3):313-7 PMID: 6291655
  28. Intracellular Ca2+ activates a fast voltage-sensitive K+ current in vertebrate sympathetic neurones.
    Nature. 1982 Apr 22;296(5859):746-9 PMID: 6280066
  29. Voltage-gated potassium channels are required for human T lymphocyte activation.
    J Exp Med. 1984 Aug 1;160(2):369-85 PMID: 6088661
  30. Ion channels in lymphocytes.
    J Clin Immunol. 1985 Jan;5(1):1-6 PMID: 2579969
  31. Macrophage membrane potential changes associated with gamma 2b/gamma 1 Fc receptor-ligand binding.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1357-61 PMID: 6219390
  32. Nonlinear current-voltage relationships in cultured macrophages.
    J Cell Biol. 1980 Apr;85(1):160-5 PMID: 7364871
  33. Functional channel formation associated with cytotoxic T-cell granules.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):150-4 PMID: 2417234
  34. Transmembrane ion fluxes during activation of human T lymphocytes: role of Ca2+, Na+/H+ exchange and phospholipid turnover.
    Immunol Rev. 1987 Feb;95:59-87 PMID: 2437015
  35. Initial characterization of the calcium channel activated by the cross-linking of the receptors for immunoglobulin E.
    J Biol Chem. 1984 Aug 25;259(16):10188-93 PMID: 6088486
  36. Evidence for anion channels in secretory vesicles.
    Neuroscience. 1988 Jun;25(3):1035-9 PMID: 2457183
  37. Electrophysiology of phagocytic membranes: induction of slow membrane hyperpolarizations in macrophages and macrophage polykaryons by intracellular calcium injection.
    J Membr Biol. 1981;61(2):81-90 PMID: 7024552
  38. The interleukin 2 receptor.
    Adv Immunol. 1988;42:165-79 PMID: 3129919
  39. The role of K+ in the regulation of the increase in intracellular Ca2+ mediated by the T lymphocyte antigen receptor.
    Cell. 1987 Jul 3;50(1):119-27 PMID: 2439211
  40. Cl- -channels in the apical cell membrane of the rectal gland "induced" by cAMP.
    Pflugers Arch. 1985 Apr;403(4):446-8 PMID: 2409518
  41. Direct induction of lymphocyte killing by calcium ionophore and phorbol ester treatment.
    Cell Immunol. 1987 Mar;105(1):23-32 PMID: 3102081
  42. Amiloride-induced suppression of lymphocyte proliferation: inhibition of IL 2 receptor expression after blockade of early sodium influx.
    Immunobiology. 1987 Aug;174(4-5):365-79 PMID: 2445661
  43. Currents carried by monovalent cations through calcium channels in mouse neoplastic B lymphocytes.
    J Physiol. 1985 Jan;358:255-84 PMID: 2580082
  44. B cell activation. I. Anti-immunoglobulin-induced receptor cross-linking results in a decrease in the plasma membrane potential of murine B lymphocytes.
    J Exp Med. 1983 Jun 1;157(6):2073-86 PMID: 6602204
  45. Action potentials in macrophages derived from human monocytes.
    Science. 1983 Feb 25;219(4587):991-3 PMID: 6823563
  46. Detection of ligand-activated conductive Ca2+ channels in human B lymphocytes.
    Cell. 1988 Jul 15;54(2):229-34 PMID: 2455602
  47. Purification and characterization of a murine cellular retinoic acid-binding protein.
    Biochem Cell Biol. 1988 Jul;66(7):750-7 PMID: 2846012
  48. K channels are expressed early in human T-cell development.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5625-9 PMID: 2426701
  49. Activation of Ca2+-dependent K+ channels in human B lymphocytes by anti-immunoglobulin.
    J Clin Invest. 1988 Feb;81(2):449-54 PMID: 2448342
  50. Ion channels activated by specific Ti or T3 antibodies in plasma membranes of human T cells.
    EMBO J. 1987 Jul;6(7):1935-9 PMID: 2443346
  51. Anion channels with multiple conductance levels in a mouse B lymphocyte cell line.
    J Physiol. 1989 Mar;410:67-90 PMID: 2477528
  52. T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes.
    Nature. 1982 Jan 7;295(5844):68-71 PMID: 6799829
  53. Role for mouse macrophage IgG Fc receptor as ligand-dependent ion channel.
    Nature. 1983 Nov 10-16;306(5939):186-9 PMID: 6316144
  54. Calcium- and voltage-activated potassium channels in human macrophages.
    Biophys J. 1984 Dec;46(6):821-5 PMID: 6097318
  55. Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscle.
    Biophys J. 1985 Jan;47(1):119-23 PMID: 2579683
  56. A cAMP-regulated chloride channel in lymphocytes that is affected in cystic fibrosis.
    Science. 1989 Feb 3;243(4891):657-60 PMID: 2464852
  57. Voltage-gated Ca2+ channel in mouse myeloma cells.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2240-2 PMID: 6300901
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869098
Published
1990-03-00
Pages
175-88
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NCI NIH HHS · CA23018 · United States
NIGMS NIH HHS · GM37767 · United States
External Links
PubMed source DOI: 10.1007/BF01869098 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2