Home LiteratureArticle Details

PMID: 2579969 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S. Review

Ion channels in lymphocytes.

Journal of clinical immunology ·Vol. 5 ·No. 1 ·1985-01-00 ·Pages 1-6

Chandy KG, DeCoursey TE, Cahalan MD, Gupta S

Abstract

The advent of the gigaohm-seal recording technique has enabled the study of the electrical properties of small cells, such as individual lymphocytes. Recent studies using this technique in combination with standard immunological and biochemical techniques indicate that cells of the immune system may utilize ion channels, similar in properties to those described in nerve and muscle, in the process of activation. For example, potassium channels may be required for T-lymphocyte mitogenesis and calcium channels for antibody production. This article summarizes these recent reports.

MeSH Terms

Animals Calcium/metabolism Cytotoxicity, Immunologic Humans In Vitro Techniques Ion Channels/metabolism Lupus Erythematosus, Systemic/metabolism Lymphocyte Activation Lymphocytes/immunology,metabolism Mice Mitogens/pharmacology Osmosis Potassium/metabolism Sodium/metabolism

Chemicals

Ion Channels Mitogens Sodium Potassium Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chandy K G

DeCoursey T E

Cahalan M D

Gupta S

References (42)

42 references, click to expand

Potasssium transport in human blood lymphocytes treated with phytohemagglutinin.
J Clin Invest. 1976 Dec;58(6):1358-69 PMID: 993349
Ionic events during the volume response of human peripheral blood lymphocytes to hypotonic media. I. Distinctions between volume-activated Cl- and K+ conductance pathways.
J Gen Physiol. 1984 Apr;83(4):497-512 PMID: 6202824
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
Pflugers Arch. 1981 Aug;391(2):85-100 PMID: 6270629
Inhibition by isoptin (a calcium antagonist) of the mitogenic stimulation of lymphocytes prior to the S-phase.
Immunology. 1978 Feb;34(2):303-7 PMID: 627409
A voltage-gated potassium channel in human T lymphocytes.
J Physiol. 1985 Jan;358:197-237 PMID: 2580081
Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes.
J Exp Med. 1979 Feb 1;149(2):516-34 PMID: 762500
Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis?
Nature. 1984 Feb 2-8;307(5950):465-8 PMID: 6320007
The early uptake of radioactive calcium by human lymphocytes treated with phytohaemagglutinin.
Immunology. 1971 Sep;21(3):509-16 PMID: 5568327
Activation-inactivation of potassium channels and development of the potassium-channel spike in internally perfused squid giant axons.
J Gen Physiol. 1981 Jul;78(1):43-61 PMID: 6265593
Volume-induced anion conductance in human B lymphocytes is cation independent.
Am J Physiol. 1983 Jul;245(1):C160-3 PMID: 6191575
Post recognition ion dependent events in mitogen induced lymphocyte proliferation and in cytotoxic effector cell responses.
Immunol Commun. 1976;5(6):517-34 PMID: 825446
T-cell mediated suppression in the MRL mouse.
Cell Immunol. 1981 Jun;61(1):40-51 PMID: 6455202
Is an early calcium flux necessary to stimulate lymphocytes?
Nature. 1977 Jun 9;267(5611):490-4 PMID: 327331
Accelerated 86Rb+ (K+) release from the cytotoxic T lymphocyte is a physiologic event associated with delivery of the lethal hit.
J Immunol. 1983 Sep;131(3):1138-41 PMID: 6604088
Deficient interleukin 2 activity in MRL/Mp and C57BL/6J mice bearing the lpr gene.
J Exp Med. 1981 Nov 1;154(5):1671-80 PMID: 6975351
Effect of phytohaemagglutinin on the surface charge of human and murine lymphocytes.
Nature. 1967 Nov 18;216(5116):694-5 PMID: 11396506
Membrane cation transport and the control of proliferation of mammalian cells.
Annu Rev Physiol. 1978;40:19-41 PMID: 147644
Estimation of membrane potentials of individual lymphocytes by flow cytometry.
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5728-30 PMID: 93281
Voltage-gated potassium channels are required for human T lymphocyte activation.
J Exp Med. 1984 Aug 1;160(2):369-85 PMID: 6088661
Mitogenic properties of a calcium ionophore, A23187.
Proc Natl Acad Sci U S A. 1974 Dec;71(12):5088-90 PMID: 4531040
Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes.
J Biol Chem. 1983 Apr 25;258(8):4876-82 PMID: 6601105
Enhanced uptake of calcium by transforming lymphocytes.
Cell Immunol. 1972 Sep;5(1):137-47 PMID: 4341425
Flux of 86Rb in activated human lymphocytes.
Can J Biochem. 1977 Jul;55(7):774-8 PMID: 890573
Early stimulation of potassium uptake in lymphocytes treated with PHA.
Exp Cell Res. 1970 Nov;63(1):230-3 PMID: 5496279
K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion.
Nature. 1984 Feb 2-8;307(5950):468-71 PMID: 6320008
Early biochemical events in lymphocyte activation. I. Investigations on the nature and significance of early calcium fluxes observed in mitogen-induced T and B lymphocytes.
Cell Immunol. 1979 May;44(2):290-313 PMID: 378421
Calcium and lymphocyte activation.
Cell Immunol. 1976 Jul;25(1):74-89 PMID: 183895
Duration of the calcium signal in the mitogenic stimulation of thymocytes.
Biochem J. 1983 Aug 15;214(2):575-9 PMID: 6604526
Membrane potential changes during mitogenic stimulation of mouse spleen lymphocytes.
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2200-4 PMID: 6929546
Potassium current in clonal cytotoxic T lymphocytes from the mouse.
J Physiol. 1984 Jun;351:645-56 PMID: 6611410
Development of a delayed outward-rectifying K+ conductance in cultured mouse peritoneal macrophages.
Proc Natl Acad Sci U S A. 1984 May;81(10):3083-7 PMID: 6328495
A calcium hypothesis for the control of cell growth.
Biochem Soc Symp. 1980;45:1-26 PMID: 6783043
Early plasma-membrane-potential changes during stimulation of lymphocytes by concanavalin A.
Biochem J. 1983 Mar 15;210(3):885-91 PMID: 6409082
Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes.
J Clin Invest. 1979 Sep;64(3):834-41 PMID: 224078
Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin.
J Cell Physiol. 1976 Mar;87(3):337-43 PMID: 1082887
Volume regulation by human lymphocytes. Role of calcium.
J Gen Physiol. 1982 May;79(5):849-68 PMID: 6808083
Role of T3 surface molecules in human T-cell activation: T3-dependent activation results in an increase in cytoplasmic free calcium.
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4169-73 PMID: 6234599
Induction of increased calcium uptake in mouse T lymphocytes by concanavalin A and its modulation by cyclic nucleotides.
Nature. 1975 May 29;255(5507):378-82 PMID: 165431
T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes.
Nature. 1982 Jan 7;295(5844):68-71 PMID: 6799829
Volume regulation by human lymphocytes. Identification of differences between the two major lymphocyte subpopulations.
J Clin Invest. 1982 Sep;70(3):632-8 PMID: 6980902
Voltage-gated Ca2+ channel in mouse myeloma cells.
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2240-2 PMID: 6300901
Increased cationic fluxes in stimulated lymphocytes of the mouse: response of enriched B- and T-cell subpopulations to B- and T-cell mitogens.
Can J Biochem. 1980 Oct;58(10):831-9 PMID: 6970062

Article Info

Journal

Journal of clinical immunology

Abbr.

J Clin Immunol

ISSN

0271-9142

DOI

10.1007/BF00915161

Published

1985-01-00

Pages

1-6

Language

English

Region

Netherlands

NLM ID

8102137

Subset

Grants

NIA NIH HHS · AG 04361 · United States

NIAID NIH HHS · AI-20717 · United States

NINDS NIH HHS · NS 14509 · United States

External Links

PubMed source DOI: 10.1007/BF00915161 View journal details

Analysis Services

Ion channels in lymphocytes.

Contact

WeChat Official Account