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PMID: 1694012 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei.

Molecular and cellular biology ·Vol. 10 ·No. 7 ·1990-07-00 ·Pages 3492-504

Rudenko G, Le Blancq S, Smith J, Lee MG, Rattray A, Van der Ploeg LH

Abstract

At least one of the procyclic acidic repetitive protein (PARP or procyclin) loci of Trypanosoma brucei is a small (5- to 6-kilobase) polycistronic transcription unit which is transcribed in an alpha-amanitin-resistant manner. Its single promoter, as mapped by run-on transcription analysis and UV inactivation of transcription, is located immediately upstream of the first alpha-PARP gene. Transcription termination occurs in a region approximately 3 kilobases downstream of the beta-PARP gene. The location of the promoter was confirmed by its ability to direct transcription of the bacterial chloramphenicol acetyltransferase gene in insect-form (procyclic) T. brucei. The putative PARP promoter is located in the region between the 3' splice acceptor site (nucleotide position 0) and nucleotide position -196 upstream of the alpha-PARP genes. Regulatory regions influencing the levels of PARP expression may be located further upstream. We conclude that a single promoter, which is located very close to the 3' splice acceptor site of the alpha-PARP genes, directs the transcription of a small, polycistronic, and alpha-amanitin-resistant transcription unit.

MeSH Terms
Amanitins/pharmacology Animals Antigens, Protozoan/genetics Base Sequence Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/genetics DNA/genetics Dose-Response Relationship, Radiation Drug Resistance/genetics Genes Membrane Glycoproteins Molecular Sequence Data Promoter Regions, Genetic Protozoan Proteins RNA/genetics,isolation & purification Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic/radiation effects Transfection Trypanosoma brucei brucei/genetics Ultraviolet Rays Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Amanitins Antigens, Protozoan Membrane Glycoproteins Protozoan Proteins Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma RNA DNA Chloramphenicol O-Acetyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rudenko G
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Le Blancq S
Smith J
Lee M G
Rattray A
Van der Ploeg L H
References (48)
48 references, click to expand
  1. The procyclic acidic repetitive proteins of Trypanosoma brucei. Purification and post-translational modification.
    J Biol Chem. 1989 Sep 5;264(25):15088-93 PMID: 2475493
  2. Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei.
    Nature. 1987 Jan 15-21;325(6101):272-4 PMID: 3808022
  3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  4. Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9119-23 PMID: 2594753
  5. Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei.
    EMBO J. 1989 Dec 20;8(13):4259-63 PMID: 2591373
  6. c-Ha-ras gene bidirectional promoter expressed in vitro: location and regulation.
    Mol Cell Biol. 1989 Sep;9(9):3758-70 PMID: 2674682
  7. Frequent independent duplicative transpositions activate a single VSG gene.
    Mol Cell Biol. 1987 Jan;7(1):357-64 PMID: 3031467
  8. A simple phase-extraction assay for chloramphenicol acyltransferase activity.
    Gene. 1988 Jul 30;67(2):271-7 PMID: 3169576
  9. Expression of a bacterial gene in a trypanosomatid protozoan.
    Science. 1989 Jun 9;244(4909):1167-9 PMID: 2499047
  10. Structure and sequence of genes encoding subunits of eukaryotic RNA polymerases.
    Oxf Surv Eukaryot Genes. 1988;5:91-131 PMID: 2472153
  11. Isolation of RNA using guanidinium salts.
    Methods Enzymol. 1987;152:219-27 PMID: 2443794
  12. Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.
    Nucleic Acids Res. 1986 Dec 9;14(23):9471-89 PMID: 3797245
  13. Discontinuous transcription and splicing in trypanosomes.
    Cell. 1986 Nov 21;47(4):479-80 PMID: 3779833
  14. The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides.
    Nucleic Acids Res. 1986 Jul 25;14(14):5605-14 PMID: 3737413
  15. Characterization of the Trypanosoma brucei 5S ribosomal RNA gene and transcript: the 5S rRNA is a spliced-leader-independent species.
    Gene. 1985;35(1-2):131-41 PMID: 4029619
  16. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  17. Comparison of the genes coding for the common 5' terminal sequence of messenger RNAs in three trypanosome species.
    Nucleic Acids Res. 1984 Jun 11;12(11):4431-43 PMID: 6204273
  18. Structure of the growing telomeres of Trypanosomes.
    Cell. 1984 Feb;36(2):459-68 PMID: 6319026
  19. Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei.
    Cell. 1983 Jan;32(1):35-43 PMID: 6825172
  20. Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication.
    Acta Trop. 1979 Sep;36(3):289-92 PMID: 43092
  21. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  22. Transcription unit mapping in bacteriophage T7. I. In vivo transcription by Escherichia coli RNA polymerase.
    J Virol. 1973 Oct;12(4):882-6 PMID: 4591054
  23. The transcriptional organization of the ribosomal RNA genes in mouse L cells.
    J Mol Biol. 1975 Jan 25;91(3):235-56 PMID: 1237628
  24. Radiological mapping of the ribosomal RNA transcription unit in E. coli.
    Nature. 1974 Oct 18;251(5476):639-41 PMID: 4608761
  25. The effects of ultraviolet irradiation on the transcription of T4 DNA.
    Biochim Biophys Acta. 1970;209(2):368-86 PMID: 5453524
  26. Discontinuous transcription and antigenic variation in trypanosomes.
    Annu Rev Biochem. 1986;55:701-32 PMID: 2874767
  27. A modular system for the assay of transcription regulatory signals: the sequence TAATGARAT is required for herpes simplex virus immediate early gene activation.
    Nucleic Acids Res. 1985 Nov 11;13(21):7847-63 PMID: 2999706
  28. Polymorphism in the procyclic acidic repetitive protein gene family of Trypanosoma brucei.
    Mol Cell Biol. 1988 Oct;8(10):4055-62 PMID: 3185541
  29. The molecular biology of the Kinetoplastidae.
    Genet Eng. 1988;(7):1-56 PMID: 2483088
  30. Procyclin: an unusual immunodominant glycoprotein surface antigen from the procyclic stage of African trypanosomes.
    Mol Biochem Parasitol. 1988 Dec;31(3):203-16 PMID: 2464763
  31. Post-transcriptional control of the differential expression of phosphoglycerate kinase genes in Trypanosoma brucei.
    J Mol Biol. 1988 May 20;201(2):315-25 PMID: 2458474
  32. The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei.
    Cell. 1987 Oct 23;51(2):261-72 PMID: 2444341
  33. VSG gene 118 is transcribed from a cotransposed pol I-like promoter.
    Cell. 1987 Aug 14;50(4):603-12 PMID: 2440585
  34. Mature mRNAs of Trypanosoma brucei possess a 5' cap acquired by discontinuous RNA synthesis.
    Nucleic Acids Res. 1985 Jun 25;13(12):4253-66 PMID: 2409534
  35. Duplication and transcription of procyclin genes in Trypanosoma brucei.
    Nucleic Acids Res. 1989 Nov 11;17(21):8727-39 PMID: 2573878
  36. Transcription of telomere repeats in protozoa.
    EMBO J. 1989 Sep;8(9):2633-8 PMID: 2511008
  37. Pseudogenes, chimaeric genes and the timing of antigen variation in African trypanosomes.
    Trends Genet. 1989 Dec;5(12):389-91 PMID: 2696181
  38. Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei.
    J Cell Biol. 1989 Feb;108(2):737-46 PMID: 2645304
  39. Variation of tandem repeats in the developmentally regulated procyclic acidic repetitive proteins of Trypanosoma brucei.
    Mol Cell Biol. 1989 Mar;9(3):1332-5 PMID: 2725502
  40. The genes and transcripts of an antigen gene expression site from T. brucei.
    Cell. 1989 Jun 2;57(5):835-45 PMID: 2720787
  41. In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure.
    Cell. 1989 Mar 10;56(5):815-27 PMID: 2924350
  42. Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain.
    Cell. 1989 Feb 24;56(4):585-97 PMID: 2917367
  43. Stable variant-specific transcripts of the variant cell surface glycoprotein gene 1.8 expression site in Trypanosoma brucei.
    Mol Cell Biol. 1988 Feb;8(2):854-9 PMID: 3352606
  44. Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes.
    Nucleic Acids Res. 1984 Dec 21;12(24):9457-72 PMID: 6514580
  45. Developmental regulation of a novel repetitive protein of Trypanosoma brucei.
    Mol Cell Biol. 1987 Aug;7(8):2838-44 PMID: 3670296
  46. Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene.
    Cell. 1987 Oct 23;51(2):273-81 PMID: 3664637
  47. Trans splicing: variation on a familiar theme?
    Cell. 1987 Jul 17;50(2):147-8 PMID: 3594568
  48. The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's.
    Science. 1986 Aug 1;233(4763):569-71 PMID: 3523758
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.7.3492-3504.1990
Published
1990-07-00
Pages
3492-504
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360784
Subset
IM
Grants
NCI NIH HHS · 5T32CA09503-05 · United States
NIAID NIH HHS · AI 21784 · United States
Databases
GENBANK
M38222, M38223, M38224
External Links
PubMed source DOI: 10.1128/mcb.10.7.3492-3504.1990 PMC Full Text View journal details
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