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PMID: 1698610 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Soluble forms of tumor necrosis factor receptors (TNF-Rs). The cDNA for the type I TNF-R, cloned using amino acid sequence data of its soluble form, encodes both the cell surface and a soluble form of the receptor.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3269-78

Nophar Y, Kemper O, Brakebusch C, Englemann H, Zwang R, Aderka D, Holtmann H, Wallach D

Abstract

Two proteins which specifically bind tumor necrosis factor (TNF) have recently been isolated from human urine in our laboratory. The two proteins cross-react immunologically with two species of cell surface TNF receptors (TNF-R). Antibodies against one of the two TNF binding proteins (TBPI) were found to have effects characteristic of TNF, including stimulating phosphorylation of specific cellular proteins. Oligonucleotide probes designed on the basis of the NH2-terminal amino acid sequence of TBPI were used to clone the cDNA for the structurally related cell surface type 1 TNF-R. It is notable that although this receptor can signal the phosphorylation of cellular proteins, it appears from its amino acid sequence to be devoid of intrinsic protein kinase activity. The extracellular domain of the receptor is composed of four internal cysteine-rich repeats, homologous to structures repeated four times in the extracellular domains of the nerve growth factor receptor and the B lymphocytes surface antigen CDw40. The amino acid composition and size of the extracellular domain of the type I TNF-R closely resemble those of TBPI. The COOH-terminal amino acid sequence of the four cysteine rich repeats within the extracellular domain of the type I TNF-R matches the COOH-terminal sequence of TBPI. Amino acid sequences in the extracellular domain also fully match other sequences found in TBPI. On the other hand, amino acid sequences in the soluble form of the type II TNF-R (TBPII), while indicating a marked homology of structure, did not suggest any identity between this protein and the extracellular domain of the type I TNF-R. CHO cells transfected with type I TNF-R cDNA produced both cell surface and soluble forms of the receptor. The receptor produced by CHO cells was recognized by several monoclonal antibodies against TBPI, reacting with several distinct epitopes in this molecule. These data suggest that the soluble forms of the TNF-Rs are structurally identical to the extracellular cytokine binding domains of these receptors and are consistent with the notion that the soluble forms are, at least partly, derived from the same transcripts that encode the cell surface receptors.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cell Membrane/metabolism Cloning, Molecular/methods Cytosol/metabolism DNA/genetics,isolation & purification Gene Library Humans Kinetics Molecular Sequence Data Oligonucleotide Probes Protein Conformation RNA/genetics,isolation & purification Receptors, Cell Surface/analysis,genetics,metabolism Receptors, Tumor Necrosis Factor Sequence Homology, Nucleic Acid Transfection Tumor Necrosis Factor-alpha/metabolism
Chemicals
Oligonucleotide Probes Receptors, Cell Surface Receptors, Tumor Necrosis Factor Tumor Necrosis Factor-alpha RNA DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Nophar Y
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
Kemper O
Brakebusch C
Englemann H
Zwang R
Aderka D
Holtmann H
Wallach D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3269-78
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552060
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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