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PMID: 1708379 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis.

Journal of bacteriology ·Vol. 173 ·No. 9 ·1991-05-00 ·Pages 2823-32

Mandrell RE, Kim JJ, John CM, Gibson BW, Sugai JV, Apicella MA, Griffiss JM, Yamasaki R

Abstract

Monoclonal antibodies (MAb) 3F11 and 06B4 recognize epitopes that are conserved on gonococcal lipooligosaccharides (LOS), present on some meningococcal LOS, and conserved on human erythrocytes. LOS of some group B and C prototype meningococcal LOS strains (LOS serotypes L1 to L8) treated with neuraminidase showed increased expression of the 3F11 and 06B4 MAb-defined epitopes. Neuraminidase-treated LOS separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver stained showed a shift in migration from a component with a mass of approximately 4.8 kDa to a component with a mass of between 4.5 and 4.6 kDa. The same strains grown in medium with excess CMP-N-acetylneuraminic acid had LOS that shifted in migration to a slightly higher component (mass, approximately 4.8 kDa). Chemical analysis of the neuraminidase-digested products from one LOS indicated it contained approximately 1.5% sialic acid. Covalent linkage between sialic acid and the LOS was confirmed by analysis of de-O-acylated and dephosphorylated LOS by liquid secondary ion mass spectrometry. Three studies show that some meningococci contain sialic acid in their LOS, that the sialic acid is cleaved and lost in conventional acetic acid hydrolysis, and that the sialic acid alters the expression of MAb-defined epitopes.

MeSH Terms
Antibodies, Monoclonal Antigens, Bacterial/metabolism Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Epitopes/analysis Gene Expression Regulation, Bacterial Lipopolysaccharides/metabolism Mass Spectrometry Microscopy, Immunoelectron N-Acetylneuraminic Acid Neisseria meningitidis/immunology Neuraminidase/pharmacology Sialic Acids/analysis
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Epitopes Lipopolysaccharides Sialic Acids lipid-linked oligosaccharides Neuraminidase N-Acetylneuraminic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Mandrell R E
Center for Immunochemistry, Veterans Administration Medical Center, San Francisco, California 94121.
Kim J J
John C M
Gibson B W
Sugai J V
Apicella M A
Griffiss J M
Yamasaki R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.9.2823-2832.1991
Published
1991-05-00
Pages
2823-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207863
Subset
IM
Grants
NIAID NIH HHS · AI 18384 · United States
NIAID NIH HHS · AI 21620 · United States
NIAID NIH HHS · AI 28871 · United States
External Links
PubMed source DOI: 10.1128/jb.173.9.2823-2832.1991 PMC Full Text View journal details
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