Home LiteratureArticle Details
PMID: 1710025 Published · ppublish English Comparative Study Journal Article

In vitro analysis of the tissue plasminogen activator promoter reveals a GC box-binding activity present in murine brain but undetectable in kidney and liver.

Molecular and cellular biology ·Vol. 11 ·No. 6 ·1991-06-00 ·Pages 3139-47

Pecorino LT, Darrow AL, Strickland S

Abstract

Tissue plasminogen activator (t-PA) mRNA levels are high in murine brain, lower in kidney, and undetectable in liver. Differences in t-PA mRNA levels are regulated in part at the transcriptional level. Brain, kidney, and liver nuclear extracts direct regulated transcription from the murine t-PA promoter in a manner that reflects the relative levels of t-PA gene expression in these tissues in vivo. Analysis of mutants has defined two GC box motifs as important elements for regulated transcription in vitro. Upon investigation of protein-DNA binding, we detected an activity in brain extracts which was not detected in kidney or liver extracts. An Sp1-like factor also binds to this region in all three tissue types. DNA interference experiments show that the brain-enriched binding activity and the Sp1-like factor contact the same GC-rich sequences. These studies provide additional evidence that brain-enriched DNA-binding activities can interact with sequences also recognized by ubiquitous transcription factors.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Northern Brain/enzymology Cell Nucleus/physiology DNA/genetics Gene Expression Regulation, Enzymologic Kidney/enzymology Liver/enzymology Mice Mice, Inbred Strains Molecular Sequence Data Nucleotide Mapping Organ Specificity Promoter Regions, Genetic RNA/genetics,isolation & purification RNA, Messenger/analysis,genetics Sequence Homology, Nucleic Acid Tissue Plasminogen Activator/genetics Transcription, Genetic
Chemicals
RNA, Messenger RNA DNA Tissue Plasminogen Activator
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pecorino L T
Program in Cellular and Developmental Biology, State University of New York, Stony Brook 11794-8651.
Darrow A L
Strickland S
References (47)
47 references, click to expand
  1. The role of cis-acting promoter elements in tissue-specific albumin gene expression.
    Science. 1989 Apr 21;244(4902):343-6 PMID: 2711183
  2. Alpha 4, the major regulatory protein of herpes simplex virus type 1, is stably and specifically associated with promoter-regulatory domains of alpha genes and of selected other viral genes.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3218-22 PMID: 3010285
  3. Nuclear factor ETF specifically stimulates transcription from promoters without a TATA box.
    J Biol Chem. 1989 Sep 15;264(26):15508-14 PMID: 2768275
  4. Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
    Cell. 1988 Dec 2;55(5):887-98 PMID: 3142690
  5. The brain-specific gene for rat aldolase C possesses an unusual housekeeping-type promoter.
    Eur J Biochem. 1989 Apr 15;181(1):33-9 PMID: 2714281
  6. Differentiation-responsive elements in the 5' region of the mouse tissue plasminogen activator gene confer two-stage regulation by retinoic acid and cyclic AMP in teratocarcinoma cells.
    Mol Cell Biol. 1989 Apr;9(4):1691-704 PMID: 2542775
  7. Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.
    Science. 1988 Mar 4;239(4844):1137-9 PMID: 2830672
  8. Tissue plasminogen activator mRNA in murine tissues.
    FEBS Lett. 1988 Feb 29;229(1):100-6 PMID: 2831081
  9. Molecular cloning of complementary DNA to mouse tissue plasminogen activator mRNA and its expression during F9 teratocarcinoma cell differentiation.
    J Biol Chem. 1988 Jan 25;263(3):1563-9 PMID: 2826484
  10. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
    Genes Dev. 1989 Dec;3(12B):2083-90 PMID: 2516827
  11. Purification of nuclear factor I by DNA recognition site affinity chromatography.
    J Biol Chem. 1986 Jan 25;261(3):1398-408 PMID: 3003068
  12. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  13. Characterization of Drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene.
    Cell. 1985 Jul;41(3):965-77 PMID: 3159479
  14. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7857-61 PMID: 3141919
  15. Behind the Fos and Jun leucine zipper.
    Cancer Cells. 1989 Nov;1(3):71-6 PMID: 2518283
  16. DNA binding site of the growth factor-inducible protein Zif268.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8737-41 PMID: 2510170
  17. A brain-specific transcription activator.
    Neuron. 1989 Nov;3(5):563-72 PMID: 2701844
  18. A glycosylated liver-specific transcription factor stimulates transcription of the albumin gene.
    Cell. 1989 Jun 30;57(7):1179-87 PMID: 2736625
  19. A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene.
    EMBO J. 1988 Dec 1;7(12):3763-70 PMID: 3208749
  20. Temporal pattern of alcohol dehydrogenase gene transcription reproduced by Drosophila stage-specific embryonic extracts.
    Nature. 1988 Feb 4;331(6155):410-5 PMID: 3340187
  21. Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues.
    Nucleic Acids Res. 1984 Sep 25;12(18):6951-63 PMID: 6548307
  22. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  23. The interplay of DNA-binding proteins on the promoter of the mouse albumin gene.
    Cell. 1987 Dec 24;51(6):963-73 PMID: 3690666
  24. Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene.
    Mol Cell Biol. 1987 Jan;7(1):305-13 PMID: 3561391
  25. Tissue-specific in vitro transcription from the mouse albumin promoter.
    Cell. 1986 Dec 5;47(5):767-76 PMID: 3779841
  26. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4394-8 PMID: 3925456
  27. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  28. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  29. Cell type-specific protein-DNA interactions in the human zeta-globin upstream promoter region: displacement of Sp1 by the erythroid cell-specific factor NF-E1.
    Mol Cell Biol. 1990 Jan;10(1):282-94 PMID: 2403638
  30. Transcription factor LSF binds two variant bipartite sites within the SV40 late promoter.
    Genes Dev. 1990 Feb;4(2):287-98 PMID: 2159933
  31. The progesterone receptor stimulates cell-free transcription by enhancing the formation of a stable preinitiation complex.
    Cell. 1990 Jan 26;60(2):247-57 PMID: 2153462
  32. Mechanism of transcriptional activation by Sp1: evidence for coactivators.
    Cell. 1990 Jun 29;61(7):1187-97 PMID: 2194667
  33. Transcription factor Sp1 is important for retinoic acid-induced expression of the tissue plasminogen activator gene during F9 teratocarcinoma cell differentiation.
    Mol Cell Biol. 1990 Nov;10(11):5883-93 PMID: 2172788
  34. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  35. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  36. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  37. Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 PMID: 6987648
  38. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  39. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  40. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  41. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  42. A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
    Cell. 1984 Feb;36(2):357-69 PMID: 6537904
  43. The genetic behaviour of a cloned mouse ribosomal DNA segment mimics mouse ribosomal gene evolution.
    J Mol Biol. 1979 Nov 15;134(4):743-63 PMID: 231673
  44. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  45. A 100-kD HeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer.
    Genes Dev. 1987 Dec;1(10):1147-60 PMID: 2828167
  46. Cellular transcription factor binds to adenovirus early region promoters and to a cyclic AMP response element.
    J Virol. 1988 Aug;62(8):3003-13 PMID: 2839717
  47. Molecular cloning and characterization of a human DNA binding factor that represses transcription.
    Cell. 1989 Dec 1;59(5):815-25 PMID: 2556218
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.6.3139-3147.1991
Published
1991-06-00
Pages
3139-47
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360162
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.6.3139-3147.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2